Molecular detection and partial characterization of Tomato Yellow Leaf Curl Virus in Sri Lanka

Tomato Yellow Leaf Curl Virus (TYLCV) is an important plant virus on one of the economically most important vegetable crops; tomato (Lycopersicon esculentum Mill.). This had not been molecularly detected before, in Sri Lanka. TYLCV-GN-SL was isolated from apparently infected tomato plants using modi...

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Published inPakistan journal of biological sciences Vol. 15; no. 18; pp. 863 - 870
Main Authors Samarakoon, S A M C, Balasuriya, A, Rajapaksha, R G A S, Wickramarachchi, W A R T
Format Journal Article
LanguageEnglish
Published Pakistan 15.09.2012
Subjects
Adenylate cyclase
Base Sequence
Begomovirus - classification
Begomovirus - genetics
DNA Primers
Geminivirus
Genes, Viral
Lycopersicon esculentum - virology
Phylogeny
Polymerase Chain Reaction
Sri Lanka
Sri Lanka
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Summary:Tomato Yellow Leaf Curl Virus (TYLCV) is an important plant virus on one of the economically most important vegetable crops; tomato (Lycopersicon esculentum Mill.). This had not been molecularly detected before, in Sri Lanka. TYLCV-GN-SL was isolated from apparently infected tomato plants using modified Cetyltrimethyl Ammonium Bromide (CTAB) method in Gannoruwa. Associated Begomoviruses were detected using Deng 541/Deng 540 and AV 494/AC 1048 primer pairs. TYLCV was detected for the first time in tomato in Sri Lanka using P1V/P4C, TYLCV specific primer pair. Nucleotide sequence of coat protein of isolated TYLCV-GN-SL proved that the Indian strain of ToLC virus was closely related to Tomato Leaf Curl Sri Lanka Virus (TLCV-SL: 97%) and Tomato leaf curl Geminivirus (TLCGV: 93%) through direct sequencing data. TLCV-SL was confirmed as TYLCV isolate. TYLCV was molecularly detected from major tomato growing districts like Badulla, Nuwara-Eliya, Kandy and Matale in Sri Lanka.
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ISSN:1028-8880
1812-5735
DOI:10.3923/pjbs.2012.863.870