Intensifying outdoor cultivation of oleaginous microalgae in photobioreactors for simultaneous wastewater bioremediation and low-cost production of renewable biodiesel feedstocks

• Published in: Biochemical engineering journal Vol. 206; p. 109318
• Main Authors: Jehalee, Faridah, Maneechote, Wageeporn, Srinuanpan, Sirasit, Pathom-aree, Wasu, Phusunti, Neeranuch, Mouradov, Aidyn, Cheirsilp, Benjamas
• Format: Journal Article
• Language: English
• Published: Elsevier B.V 01.06.2024
• Subjects: Lipid; Outdoor cultivation; Seafood secondary effluent; Thermotolerant; Outdoor cultivation; Seafood secondary effluent; Lipid; Thermotolerant
• ISSN: 1369-703X; 1873-295X
• DOI: 10.1016/j.bej.2024.109318

Oleaginous microalgae are gaining increasing attention for simultaneous wastewater bioremediation and biofuel feedstock production. In this study, four oleaginous species of microalgae including Chlamydomonas sp. WWP, Scenedesmus sp. HP56, Haematococcus sp., and Nannochloropsis sp., were screened for their growth and lipid production at 30, 35, and 40 °C, under light intensity at 3, 4, and 5 klux. Among the strains screened, Haematococcus sp. grew best and gave comparable high growth at 30 °C and 35 °C (0.7–0.8 g/L) indicating its thermotolerant characteristic, and the optimal light intensity was found at 4 klux. Haematococcus sp. also grew well using unsterile secondary effluent from the seafood processing plant and produced high biomass (0.53–0.57 g/L) and lipids (0.17–0.19 g/L), especially in the column photobioreactor (CP). The repeated-batch outdoor cultivation mode showed that CP equipped with four-blade baffles was most efficient for the production of biomass (0.40–0.63 g/L) and lipids (0.15–0.30 g/L) during 4 cycles. The microalga also removed total nitrogen and total phosphorus from secondary effluent by 70.59% and 94.97%, respectively. The fatty acid compositions in microalgal lipids were C16-C18, especially palmitic acid, oleic acid, and linolenic acid, which show superior fuel properties, suggesting their promising use as biodiesel feedstocks. These strategies may have a significant impact not only on bioremediation of industrial effluent but also low-cost production of microalgae-based biodiesel feedstocks. [Display omitted] •Haematococcus sp. was suitable oleaginous microalga for outdoor cultivation.•Column photobioreactor with 4-blade baffles most enhanced microalgal growth.•Repeated-batch cultivation effectively produced biomass of 0.40–0.63 g/L.•Total nitrogen and total phosphorus in effluent were removed by 70.59% and 94.97%.•The microalgal lipids have high potential as biodiesel feedstocks.