Polycaprolactone nanofibers colonized by HeLa cells: an alternative for in vitro investigation of fungal infection

3D equivalent tissues can be used for studies of fungal infections. To develop 3D electrospun nanofibers using polycaprolactone (PCL) colonized by HeLa cells as a possible model for the investigation of fungal infection. A PCL solution was synthesized and electrospun. HeLa cells were cultured on the...

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Bibliographic Details
Published inFuture microbiology Vol. 18; no. 4; pp. 199 - 213
Main Authors Cruz, Elton, Diniz, Beatriz V, Ochikubo, Larissa Mk, Kawasaki, Thaís E, Veiga, Flávia F, Lazarin-Bidóia, Danielle, Cesar, Gabriel B, Radovanovic, Eduardo, Caetano, Wilker, Svidzinski, Terezinha Ie, Negri, Melyssa
Format Journal Article
LanguageEnglish
Published England 01.03.2023
Subjects
Biocompatible Materials - chemistry
HeLa Cells
Humans
Mycoses
Nanofibers - chemistry
Tissue Engineering
Tissue Scaffolds - chemistry
Candida albicans
HeLa cells
infection model
equivalent tissue
infection fungal
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Summary:3D equivalent tissues can be used for studies of fungal infections. To develop 3D electrospun nanofibers using polycaprolactone (PCL) colonized by HeLa cells as a possible model for the investigation of fungal infection. A PCL solution was synthesized and electrospun. HeLa cells were cultured on the nanostructured PCL scaffolds, forming a 3D structure. Physicochemical, biological and infection assays were performed in this model. The nanostructured PCL scaffolds showed favorable physicochemical characteristics and allowed the colonization of HeLa cells, which showed indications of extracellular matrix production. Fungal infection was evidenced in the 3D nanostructured PCL scaffolds, being viable, economical and compatible to study fungal infections .
Bibliography:ObjectType-Article-1
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content type line 23
ISSN:1746-0913
1746-0921
DOI:10.2217/fmb-2022-0017