Genetic diversity and structure of a core collection of Huangqi (Astragalus ssp.) developed using genomic simple sequence repeat markers

• Published in: Genetic resources and crop evolution Vol. 70; no. 2; pp. 571 - 585
• Main Authors: Gong, Fanshu, Geng, Yaping, Zhang, Pengfei, Zhang, Feng, Fan, Xinfeng, Liu, Yaling
• Format: Journal Article
• Language: English
• Published: Dordrecht Springer Netherlands 01.02.2023; Springer Nature B.V
• Subjects: Agriculture; Algorithms; Alleles; Biomedical and Life Sciences; Collection; Genetic analysis; Genetic diversity; Germplasm; Life Sciences; Lung diseases; Maximization; Optimization; Phylogeny; Plant Genetics and Genomics; Plant Physiology; Plant Sciences; Plant Systematics/Taxonomy/Biogeography; Population structure; Research Article; Simulated annealing; SSR; Molecular marker; Core collection; Huangqi
• ISSN: 0925-9864; 1573-5109
• DOI: 10.1007/s10722-022-01447-x

Huangqi ( Astragalus spp. ) is a versatile herb that possesses several therapeutic effects against a variety of diseases, especially lung diseases. The aim of this study was to establish a core collection of Astragalus germplasm resources based on 10 simple sequence repeat (SSR) markers. We used 380 samples of Astragalus collected from different regions to a core Astragalus collection using five different methods, including PowerCore-based M strategy, CoreFinder-based M strategy, Core Hunter-based stepwise sampling, PowerMarker-based simulated annealing algorithm based on allele maximization, and PowerMarker-based simulated annealing algorithm based on maximizing genetic diversity. Among these methods, the CoreFinder-based M strategy was found to be the most suitable approach as it preserved all the alleles, and most of the genetic diversity parameters of the constructed core collections were higher than those of the initial collection. Additional analyses demonstrated that the genetic diversity of the core collection was similar to that of the initial collection. Further, phylogenetic trees indicated that the population structure of the core collection was similar to that of the initial collection. In addition, our results showed that the optimal grouping value of K was 2. The construction of a core collection is beneficial for the understanding, management, and utilization of Astragalus . Moreover, this study will serve as a valuable reference for constructing core collections of other plants and fungi.