Cytochrome P450 monooxygenase systems: Diversity and plasticity for adaptive stress response

• Published in: Progress in biophysics and molecular biology Vol. 193; pp. 19 - 34
• Main Authors: Mokhosoev, Innokenty M., Astakhov, Dmitry V., Terentiev, Alexander A., Moldogazieva, Nurbubu T.
• Format: Journal Article
• Language: English
• Published: England Elsevier Ltd 01.11.2024
• Subjects: CYP; Cytochrome P450; diversity; plasticity; redox partners; stress adaptation; stress adaptation; plasticity; diversity; CYP; redox partners; Cytochrome P450
• ISSN: 0079-6107; 1873-1732; 1873-1732
• DOI: 10.1016/j.pbiomolbio.2024.09.003

Superfamily of cytochromes P450 (CYPs) is composed of heme-thiolate-containing monooxygenase enzymes, which play crucial roles in the biosynthesis, bioactivation, and detoxification of a variety of organic compounds, both endogenic and exogenic. Majority of CYP monooxygenase systems are multi-component and contain various redox partners, cofactors and auxiliary proteins, which contribute to their diversity in both prokaryotes and eukaryotes. Recent progress in bioinformatics and computational biology approaches make it possible to undertake whole-genome and phylogenetic analyses of CYPomes of a variety of organisms. Considerable variations in sequences within and between CYP families and high similarity in secondary and tertiary structures between all CYPs along with dramatic conformational changes in secondary structure elements of a substrate binding site during catalysis have been reported. This provides structural plasticity and substrate promiscuity, which underlie functional diversity of CYPs. Gene duplication and mutation events underlie CYP evolutionary diversity and emergence of novel selectable functions, which provide the involvement of CYPs in high adaptability to changing environmental conditions and dietary restrictions. In our review, we discuss the recent advancements and challenges in the elucidating the evolutionary origin and mechanisms underlying the CYP monooxygenase system diversity and plasticity. Our review is in the view of hypothesis that diversity of CYP monooxygenase systems is translated into the broad metabolic profiles, and this has been acquired during the long evolutionary time to provide structural plasticity leading to high adaptative capabilities to environmental stress conditions. •CYPs have acquired functions of monooxygenases during the evolution.•Diversity of CYP monooxygenase systems underlies high stress adaptability.•Conformational changes in ligand binding cavity provide substrate promiscuity.•Structural plasticity of CYPs contributes to adaptation to environmental changes.