The kinetics of induction of Hox1.6 and C-jun mRNA during three different ways of inducing differentiation in teratocarcinoma F9 cells

• Published in: In vitro cellular & developmental biology. Animal Vol. 31; no. 6; p. 462
• Main Authors: Iwai, S A, Nishina, Y, Kosaka, M, Sumi, T, Doi, T, Sakuda, M, Nishimune, Y
• Format: Journal Article
• Language: English
• Published: Germany 01.06.1995
• Subjects: Butyrates - pharmacology; Butyric Acid; Cell Differentiation - genetics; Cycloheximide - pharmacology; Gene Expression Regulation - drug effects; Homeodomain Proteins - genetics; Humans; Kinetics; Mutation; Proto-Oncogene Proteins c-jun - genetics; RNA, Messenger - metabolism; Teratocarcinoma; Transcription Factors - genetics; Tretinoin - pharmacology; Tumor Cells, Cultured
• ISSN: 1071-2690
• DOI: 10.1007/BF02634259

Changes in Hox1.6 and c-jun gene expression were examined upon F9 cell differentiation that was induced by three independent methods: a drug treatment with retinoic acid (RA), that with sodium butyrate (NaB), and a genetic approach using the ts mutant. To obtain further information on the mechanism of teratocarcinoma cell differentiation we have examined the kinetics of the induction of Hox1.6 and c-jun mRNA whose gene products have been demonstrated to have specific roles in gene regulation. Expression of Hox1.6 mRNA was induced more rapidly than c-jun mRNA by all the above three inducing methods. Furthermore, protein synthesis was not required for the induction of Hox1.6 mRNA as well as of c-jun mRNA synthesis in all three methods. The data suggested that the transcriptional increase in the Hox1.6 mRNA was a primary response and could play an important role in F9 cell differentiation.