Detection of Neuronal and Glial Antigens After Decalcification in Formic Acid Solution and Fixation in Zinc-Ethanol-Formaldehyde

The aim of the present work was to identify the possibility of immunocytochemical detection of various tissue antigens on paraffin sections of rat spinal cord surrounded by spinal bony tissue and adjacent muscles fixed in zinc-ethanol-formaldehyde after decalcification with formic acid in adult ( n...

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Published inNeuroscience and behavioral physiology Vol. 44; no. 7; pp. 790 - 792
Main Authors Kolos, E. A., Korzhevskii, D. E.
Format Journal Article
LanguageEnglish
Published Boston Springer US 01.09.2014
Springer Nature B.V
Subjects
Acids
Antigens
Behavioral Sciences
Biomedical and Life Sciences
Biomedicine
Cloning
Ethanol
Monoclonal antibodies
Morphology
Neurobiology
Neurosciences
Polymers
Proteins
Spinal cord
Denmark
United States > US
Russia
decalcification
zinc-ethanol-formaldehyde fixation
immunohistochemistry
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Summary:The aim of the present work was to identify the possibility of immunocytochemical detection of various tissue antigens on paraffin sections of rat spinal cord surrounded by spinal bony tissue and adjacent muscles fixed in zinc-ethanol-formaldehyde after decalcification with formic acid in adult ( n = 5) and neonatal ( n = 2) Wistar rats. These studies used antibodies to glial fibrillary acidic protein, neurofilaments, neuronal nuclear protein, tyrosine hydroxylase, the synaptic vesicle protein synaptophysin, and the intermediate filament protein vimentin. The combination of fixation in zinc-ethanol-formaldehyde and decalcification in 21% formic acid solution provided optimal preservation of the antigens of interest.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
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ISSN:0097-0549
1573-899X
DOI:10.1007/s11055-014-9985-3