The micronucleus test with mouse peripheral blood on N-methyl- N'-nitro- N-nitrosoguanidine and mitomycin C

The micronucleus test using mouse peripheral blood was conducted with N-methyl- N'-nitro- N-nitro-soguanidine (MNNG) and mitomycin C (MMC) as part of the 5th collaborative study supported by the Environmental Mutagen Society of Japan (CSGMT/MMS · JEMS). Male CD-1 mice were intraperitoneally inj...

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Published inMutation Research/Genetic Toxicology Vol. 278; no. 2; pp. 121 - 125
Main Authors Masahiro, Kuramochi, Hiroshi, Seki, Tadashi, Tazawa, Sayuri, Sakai, Yoshiki, Sakai
Format Journal Article
LanguageEnglish
Published Amsterdam Elsevier B.V 1992
Elsevier
Subjects
Acridine orange
Biological and medical sciences
General aspects. Methods
Medical sciences
Peripheral blood
Reticulocytes
Toxicology
Acridine orange
Reticulocytes
Peripheral blood
Performance evaluation
Vertebrata
Mammalia
Micronucleus test
Mouse
Mutagenicity testing
Cell labelling
Rodentia
Reticulocyte
Blood
Acridine dye
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Summary:The micronucleus test using mouse peripheral blood was conducted with N-methyl- N'-nitro- N-nitro-soguanidine (MNNG) and mitomycin C (MMC) as part of the 5th collaborative study supported by the Environmental Mutagen Society of Japan (CSGMT/MMS · JEMS). Male CD-1 mice were intraperitoneally injected once with 12.5–100 mg/kg of MMC. Peripheral blood was drawn at different intervals after treatment, placed on slides previously coated with acridine orange and the numbers of reticulocytes with micronuclei (MNRETs) were scored. The experiments indicated that the maximum effect of both MNNG and MMC was found about 48 h after treatment, and that the micronucleus test using peripheral blood is useful for the screening of chemicals throughout the experimental period in a single animal.
ISSN:0165-1218
DOI:10.1016/0165-1218(92)90221-K