Disinfection Efficacy and Eventual Harmful Effect of Chemical Peracetic Acid (PAA) and Probiotic Phaeobacter inhibens Tested on Isochrisys galbana (var. T-ISO) Cultures

• Published in: Water (Basel) Vol. 16; no. 16; p. 2257
• Main Authors: Casoni, Elia, Contis, Gloria, Aguiari, Leonardo, Mistri, Michele, Munari, Cristina
• Format: Journal Article
• Language: English
• Published: Basel MDPI AG 01.08.2024
• Subjects: Acids; Algae; Aquaculture; Bacteria; chemicals; Disinfection & disinfectants; Experiments; hatchery; Laboratories; microalgae; microbial pathogens; Mollusks; Pathogens; Probiotics; Salinity; Water quality
• ISSN: 2073-4441; 2073-4441
• DOI: 10.3390/w16162257

One of the main threats to aquaculture is represented by microbial pathogens, causing mass mortality episodes in hatcheries, which result in huge economic losses. Among the many disinfection methods applied to reduce this issue, the use of chemicals and beneficial microorganisms (probiotics) seems to be the most efficient. The aim of this study is to test the efficacy of two of them: a chemical, peracetic acid (PAA), and a probiotic, Phaeobacter inhibens. Tests were run on microalgae of the species Isochrysis galbana (var T-ISO). For both remedies, the microalgae survival rate and final cell concentration (cell/mL) were monitored. PAA analysis tested six different concentrations of the chemical: 7.5 µg, 10 µg/L, 20 µg/L, 30 µg/L, 40 µg/L, and 60 µg/L. Meanwhile, P. inhibens was tested with a concentration of 104 CFU/mL. Analysis for both the remedies was conducted on a laboratory scale using glass flasks, and on an industrial scale inside photobioreactors (PBRs). Among all the treatments, the one with PAA dosed with a concentration of 60 µg/L gave the best results, as the culture reached a final density of 8.61 × 106 cell/mL. However, none of the remedies involved in the experiment harmed microalgae or their growth. The results match perfectly with the condition requested for the tested remedies: to obtain an optimal breakdown of pathogens without interfering with culture growth. These features make PAA and P. inhibens good candidates for disinfection methods in aquaculture facilities.