Protein fractionation in a vortex flow filter. II: Separation of simulated mixtures

• Published in: Journal of membrane science Vol. 112; no. 1; pp. 75 - 84
• Main Authors: Balakrishnan, M., Agarwal, G.P.
• Format: Journal Article
• Language: English
• Published: Amsterdam Elsevier B.V 03.04.1996; Elsevier
• Subjects: Analytical, structural and metabolic biochemistry; Biological and medical sciences; Concentration polarization; Fundamental and applied biological sciences. Psychology; General aspects, investigation methods; Protein fractionation; Protein transmission; Proteins; Separation factor; Ultrafiltration; Vortex flow filter; Separation factor; Protein fractionation; Ultrafiltration; Vortex flow filter; Concentration polarization; Protein transmission; Myoglobin; Ovalbumin; Hemoprotein; Enzyme; Glycoproteins; Proteins; Separation method; Glycosidases; Membrane filtration; Molecular model; Hydrolases; O-Glycosidases; Lysozyme
• ISSN: 0376-7388; 1873-3123
• DOI: 10.1016/0376-7388(95)00267-7

This work investigates the fractionation of simulated mixtures of lysozyme/ovalbumin and lysozyme/myoglobin in a vortex flow ultrafilter. The operating parameters for the separations were identified from transmission studies conducted previously for each of these three proteins [3]. At low transmembrane pressure and high membrane rotation speed, the ultrafiltration characteristics of a dilute protein mixture were virtually identical to those of its individual components. The selectivity was controlled primarily by the extent of polarization of the smaller, preferentially transported species (lysozyme). Thus, high separation factors were obtained under operating conditions favoring lysozyme buildup at the membrane surface. It was further observed that better separation occurred at low fluxes (corresponding to low applied pressure) thereby indicating that the throughput and degree of resolution are mutually exclusive.