Proteomic analysis of nascent polypeptide chains that potentially induce translational pausing during elongation

ABSTRACT Currently, proteins equipped with “ribosomal arrest peptides” (RAPs) that regulate the expression of downstream genes and their own activity by pausing their own translation during elongation are extensively studied. However, studies focusing on RAP have been conducted primarily in prokaryo...

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Published inBioscience, biotechnology, and biochemistry Vol. 86; no. 9; pp. 1262 - 1269
Main Authors Shimohata, Nobuyuki, Harada, Yudai, Hayano, Toshiya
Format Journal Article
LanguageEnglish
Published 24.08.2022
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Summary:ABSTRACT Currently, proteins equipped with “ribosomal arrest peptides” (RAPs) that regulate the expression of downstream genes and their own activity by pausing their own translation during elongation are extensively studied. However, studies focusing on RAP have been conducted primarily in prokaryotic cells; studies on eukaryotic cells, especially mammalian cells, are limited. In the present study, we comprehensively examined translationally arrested nascent polypeptides to gain novel insights into RAPs in mammalian cells. Cetyltrimethylammonium bromide was used to obtain nascent polypeptide chains that were translationally arrested during translation elongation. After proteomic analysis, additional screening by discriminating according to amino acid residues at the C-terminal end revealed several novel RAP candidates. Our method can be applied for comprehensive RAP studies in mammalian cells.
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ISSN:1347-6947
1347-6947
DOI:10.1093/bbb/zbac097