Species heterogeneity of hepatic α1-adrenoceptors: α1A-, α1B- and α1C-subtypes
α 1-Adrenergic activation stimulated phosphorylase and phosphoinositide turnover in hepatocytes from guinea pigs, rats and rabbits. Chlorethylclonidine inhibited these effects in rat and rabbit cells but not in guinea pig hepatocytes; low concentrations of 5-methyl urapidil blocked the α 1 actions i...
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Published in | Biochemical and biophysical research communications Vol. 186; no. 2; pp. 760 - 767 |
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Main Authors | , , , , , |
Format | Journal Article |
Language | English |
Published |
San Diego, CA
Elsevier Inc
31.07.1992
Elsevier |
Subjects | |
Online Access | Get full text |
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Summary: | α
1-Adrenergic activation stimulated phosphorylase and phosphoinositide turnover in hepatocytes from guinea pigs, rats and rabbits. Chlorethylclonidine inhibited these effects in rat and rabbit cells but not in guinea pig hepatocytes; low concentrations of 5-methyl urapidil blocked the
α
1 actions in guinea pig and rabbit liver cells, but not in rat hepatocytes. Binding competition experiments also showed high affinity for 5-methyl urapidil in liver membranes from guinea pigs and rabbits and low affinity in those from rats. The data indicated that guinea pig hepatocytes express
α
1A-, rat hepatocytes
α
1B- and rabbit hepatocytes
α
1C-adrenoceptors. This was confirmed by Northern analysis using receptor subtype-selective probes. |
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ISSN: | 0006-291X 1090-2104 |
DOI: | 10.1016/0006-291X(92)90811-X |