Genome-Wide Profiling Identifies Saccharomyces Cerevisiae Response in PKC-SLT2 Signaling and Glycogen Metabolic Pathways to Antifungal Compound Calcofluor White

Fungal infection remains a major problem worldwide, yet treatment options are limited owing to the lack of effective drugs, the significant toxicity of available compounds, and the emergence of drug resistance. The low toxicity of calcofluor white （CFW） is an attractive antifungal compound for its k...

Full description

Saved in:
Bibliographic Details
Published inShanghai jiao tong da xue xue bao Vol. 19; no. 5; pp. 574 - 579
Main Author 周娟 胡传圣 李晓林 程酩 郭妍 邵志峰
Format Journal Article
LanguageEnglish
Published Heidelberg Shanghai Jiaotong University Press 01.10.2014
Subjects
Activated
Architecture
Candida albicans
Computer Science
Drugs
Effectiveness
Electrical Engineering
Engineering
Glycogens
Kinases
Life Sciences
Materials Science
Pathways
Proteins
Saccharomyces cerevisiae
Toxicity
Trichophyton rubrum
signaling pathway
antifungal
calcofluor white
gene expression
Q 291
Online AccessGet full text

Cover

More Information
Summary:Fungal infection remains a major problem worldwide, yet treatment options are limited owing to the lack of effective drugs, the significant toxicity of available compounds, and the emergence of drug resistance. The low toxicity of calcofluor white （CFW） is an attractive antifungal compound for its known inhibitive effects on trichophyton rubrum and candida albicans growth. However, the efficacy of CFW is limited in most cases. In order to search for effective means to improve its efficacy, using saccharomyces cerevisiae as a model, we have used microarrays to examine the cell＇s response when treated with CFW on the genome scale. We found that both the PKC-SLT2 （i.e, protein kinase C-mitogen activated protein kinase） and the glycogen metabolic pathways are activated upon CFW treatment. These results suggest that the key components in these pathways could be targeted by other drugs to counter the cell＇s compensative response, thus to further substantiate the inhibitive effect of CFW on fungal growth, which may lead to treatment regimens with improved efficacy of this compound in clinical applications.
Bibliography:Fungal infection remains a major problem worldwide, yet treatment options are limited owing to the lack of effective drugs, the significant toxicity of available compounds, and the emergence of drug resistance. The low toxicity of calcofluor white （CFW） is an attractive antifungal compound for its known inhibitive effects on trichophyton rubrum and candida albicans growth. However, the efficacy of CFW is limited in most cases. In order to search for effective means to improve its efficacy, using saccharomyces cerevisiae as a model, we have used microarrays to examine the cell＇s response when treated with CFW on the genome scale. We found that both the PKC-SLT2 （i.e, protein kinase C-mitogen activated protein kinase） and the glycogen metabolic pathways are activated upon CFW treatment. These results suggest that the key components in these pathways could be targeted by other drugs to counter the cell＇s compensative response, thus to further substantiate the inhibitive effect of CFW on fungal growth, which may lead to treatment regimens with improved efficacy of this compound in clinical applications.
31-1943/U
saccharomyces cerevisiae, antifungal, calcofluor white, gene expression, signaling pathway
ZHOU Juan, HU Chuan-sheng, LI Xiao-lin CHENG Ming, GUO Yah, SHAO Zhi-feng（a. Institute of System Biomedicine; b. Bio-IDCenter,School of Biomedical Engineering, Shanghai Jiaotong University, Shanghai 200240, China）
ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
ISSN:1007-1172
1995-8188
DOI:10.1007/s12204-014-1544-0