Identification of mRNAs with enhanced expression in ripening strawberry fruit using polymerase chain reaction differential display

Fruit ripening is a complex developmental process that involves specific changes in gene expression and cellular metabolism. In climateric fruits these events are coordinated by the gaseous hormone ethylene, which is synthesized autocatalytically in the early stages of ripening. Nonclimacteric fruit...

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Bibliographic Details
Published inPlant molecular biology Vol. 27; no. 6; p. 1097
Main Authors Wilkinson, J.Q. (Monsanto Co., Chesterfield, MO (USA). Agricultural Group), Lanahan, M.B, Conner, T.W, Klee, H.J
Format Journal Article
LanguageEnglish
Published Netherlands 01.03.1995
Subjects
Amino Acid Sequence
AMPLIFICATION CHAINE POLYMERASE
ARN MENSAJERO
ARN MESSAGER
Base Sequence
Cloning, Molecular
DNA, Complementary
EXPRESION GENICA
EXPRESSION DES GENES
FRAGARIA
Fruit - genetics
Fruit - physiology
GENE EXPRESSION
MADURAMIENTO
MESSENGER RNA
Molecular Sequence Data
MURISSAGE
POLYMERASE CHAIN REACTION
REACCION DE CADENAS DE POLIMERASA
RIPENING
RNA, Messenger - genetics
Sequence Homology, Amino Acid
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Summary:Fruit ripening is a complex developmental process that involves specific changes in gene expression and cellular metabolism. In climateric fruits these events are coordinated by the gaseous hormone ethylene, which is synthesized autocatalytically in the early stages of ripening. Nonclimacteric fruits do not synthesize or respond to ethylene in this manner, yet undergo many of the same physiological and biochemical changes associated with the production of a ripe fruit. To gain insight into the molecular determinants associated with nonclimacteric fruit ripening, we examined mRNA populations in ripening strawberry fruit using polymerase chain reaction (PCR) differential display. Five mRNAs with ripening-enhanced expression were identified using this approach. Three of the mRNAs appear to be fruit-specific, with little or no expression detected in vegetative tissues. Sequence analysis of cDNA clones revealed positive identities for three of the five mRNAs based on homology to known proteins. These results indicate that the differential display technique can be a useful tool to study fruit ripening and other developmental processes in plants at the RNA level.
Bibliography:9504282
F30
F62
ISSN:0167-4412
1573-5028
DOI:10.1007/BF00020883