Genetic evidence for the interaction of the yeast transcriptional co-activator proteins GCN5 and ADA2

• Published in: Molecular & general genetics Vol. 246; no. 6; p. 723
• Main Authors: Georgakopoulos, T, Gounalaki, N, Thireos, G. (Foundation for Research and Technology Hellas, Heraklion (Greece). Inst. of Molecular Biology and Biotechnology)
• Format: Journal Article
• Language: English
• Published: Germany 01.11.1995
• Subjects: ADA2; Bromodomain; Cloning, Molecular; DNA-Binding Proteins; Fungal Proteins - physiology; GCN5; GENE; GENES; Histone Acetyltransferases; INTERACCION DE GENES; INTERACTION GENIQUE; Mutation; Pflanzenzuechtung; Plasmids; Protein Kinases - physiology; PROTEINAS; PROTEINE; SACCHAROMYCES CEREVISIAE; Saccharomyces cerevisiae - genetics; Saccharomyces cerevisiae Proteins; Trans-Activators - physiology; Transcription Factors - physiology; Transcriptional co-activators
• ISSN: 0026-8925; 1432-1874
• DOI: 10.1007/BF00290718

The GCN5 and ADA2 proteins are required for the activation function of a number of transcriptional activators in the yeast Saccharomyces cerevisiae. By using appropriate LexA fusion proteins it is demonstrated that both proteins are required for part of the function of the GCN4, GAL4 and the VP16 transcriptional activation domains. Analysis of a gcn1 ada2 double disruption mutant did not reveal any additive effects, suggesting that the two proteins act in the same pathway. The GCN5 ,md ADA2 proteins can each activate transcription when directed to the promoter region of a reporter gene, but only in the presence of a wild-type ADA2 or GCN5 gene, respectively. The activation capacity is enhanced when the corresponding endogenous gene copy is disrupted. Taken together, these genetic data suggest that the two proteins interact and define one complex that mediates transcriptional activation. The function of this complex requires the bromodomain region of the GCN5 protein.