Key energy metabolisms in modern living microbialites from hypersaline Andean lagoons of the Salar de Atacama, Chile

• Published in: The Science of the total environment Vol. 937; p. 173469
• Main Authors: Osman, Jorge R., Castillo, Julio, Sanhueza, Vilma, Miller, Ana Z., Novoselov, Alexey, Cotoras, Davor, Morales, Daniela
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 10.08.2024
• Subjects: Environmental adaptation; Metabolic pathway; Metagenomes; Microbialites; Minerals; Environmental adaptation; Metagenomes; Metabolic pathway; Minerals; Microbialites
• ISSN: 0048-9697; 1879-1026
• DOI: 10.1016/j.scitotenv.2024.173469

Microbialites are organosedimentary structures formed mainly due to the precipitation of carbonate minerals, although they can also incorporate siliceous, phosphate, ferric, and sulfate minerals. The minerals' precipitation occurs because of local chemical changes triggered by changes in pH and redox transformations catalyzed by the microbial energy metabolisms. Here, geochemistry, metagenomics, and bioinformatics tools reveal the key energy metabolisms of microbial mats, stromatolites and an endoevaporite distributed across four hypersaline lagoons from the Salar de Atacama. Chemoautotrophic and chemoheterotrophic microorganisms seem to coexist and influence microbialite formation. The microbialite types of each lagoon host unique microbial communities and metabolisms that influence their geochemistry. Among them, photosynthetic, carbon- and nitrogen- fixing and sulfate-reducing microorganisms appear to control the main biogeochemical cycles. Genes associated with non-conventional energy pathways identified in MAGs, such as hydrogen production/consumption, arsenic oxidation/reduction, manganese oxidation and selenium reduction, also contribute to support life in microbialites. The presence of genes encoding for enzymes associated with ureolytic processes in the Cyanobacteria phylum and Gammaproteobacteria class might induce carbonate precipitation in hypersaline environments, contributing to the microbialites formation. To the best of our knowledge, this is the first study characterizing metagenomically microbialites enriched in manganese and identifying metabolic pathways associated with manganese oxidation, selenium reduction, and ureolysis in this ecosystem, which suggests that the geochemistry and bioavailability of energy sources (As, Mn and Se) shapes the microbial metabolisms in the microbialites. [Display omitted] •Metagenomic characterization of microbialites enriched in Mn•The arsenic seems to be one of the main energy pathways in these microbialites.•Genes associated to manganese oxidation and selenium reduction•Genes encoding ureases contribute to the microbialites formation.