Valorization of Potato Peels Starch for Efficient β‐Cyclodextrin Production and Purification through an Eco‐Friendly Process

• Published in: Starch - Stärke Vol. 74; no. 9-10
• Main Authors: Ben Amara, Fakhreddine, Bouzid, Moetaz, Sahnoun, Mouna, Ben Nasr, Yosri, Jaouadi, Bassem, Bejar, Samir, Jemli, Sonia
• Format: Journal Article
• Language: English
• Published: Weinheim Wiley Subscription Services, Inc 01.09.2022
• Subjects: CGTase; Crystallization; Cyclodextrins; E coli; Homogeneity; Industrial applications; Oligosaccharides; potato peels starch; Potatoes; Processing industry; Production costs; Purification; Starch; β-Cyclodextrin; β‐cyclodextrins
• ISSN: 0038-9056; 1521-379X
• DOI: 10.1002/star.202200037

Potato peels (PP) are agro‐industrial co‐products generated in huge quantities by the potato processing industry. Obtaining value‐added products from PP is a current research challenge. As cyclic oligosaccharides is produced from starch by the action of cyclodextrin glucanotransferase (CGTase), β‐cyclodextrins (β‐CDs) are increasingly used in various industrial applications. In an attempt to develop a low‐cost process for β‐CDs production, starch is firstly extracted from PP. The productions of the US132 CGTase and β‐CDs are then optimized via experimental designs. The US132 CGTase production from the recombinant T7 express Escherichia coli strain is enhanced 20‐fold to reach 59.95 U mL−1. Subsequently, the β‐CDs production by the US132 CGTase is optimized to reach a high level (67.26 g L−1). The β‐CDs are then purified to homogeneity through a simple eco‐friendly crystallization method that provided a purification yield of 20%. This study reveals that β‐CDs production from valorized starch is a promising strategy used to valorize PP and minimize β‐CDs production cost. An eco‐friendly and low‐cost process is developed for the β‐CDs production and purification. The study shows the promising characteristics of the potato peels starch that can be applied to generate a high amount of β‐CDs. Moreover, an efficient protocol of β‐CDs purification without any use of organic solvents is detailed.