Determination Methods for Vero Toxins Produced by Enterohemorrhagic Escherichia coli

During the past 20 years, outbreaks of enterohemorrhagic Escherichia coli (EHEC) have been increasing worldwide and have been recognized as a potential health concern. Vero toxins produced by EHEC seem to be the most common cause of hemolytic uremic syndrome. Rapid diagnosis of EHEC infection is imp...

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Published inJournal of the Mass Spectrometry Society of Japan Vol. 51; no. 1; pp. 114 - 118
Main Authors KONDO, Fumio, SUZUKI, Masahiro
Format Journal Article
LanguageEnglish
Published Tokyo The Mass Spectrometry Society of Japan 2003
Japan Science and Technology Agency
Subjects
Diagnosis
Electrospray ionization-liquid chromatography/mass spectrometry
Enterohemorrhagic Escherichia coli
Escherichia coli
Hemolytic uremic synolrome
Vero toxin
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Summary:During the past 20 years, outbreaks of enterohemorrhagic Escherichia coli (EHEC) have been increasing worldwide and have been recognized as a potential health concern. Vero toxins produced by EHEC seem to be the most common cause of hemolytic uremic syndrome. Rapid diagnosis of EHEC infection is important to prevent the expansion of infection. Diagnosis is carried out by both isolation of EHEC and detection of Vero toxins in fecal extracts or fecal cultures. This review describes briefly about the current knowledge of the EHEC and Vero toxins, and about the determination methods for Vero toxins. The attempt to identify Vero toxins by electrospray ionization-liquid chromatography/mass spectrometry is also discussed.
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ISSN:1340-8097
1880-4225
DOI:10.5702/massspec.51.114