An Electrochemical HbA1c Immunosensor with a Simple Electrode of Gold Nanoparticle Monolayer

• Published in: IEEE sensors journal Vol. 23; no. 15; p. 1
• Main Authors: Tseng, Chun-Chiang, Lin, Ying-Ting, Wang, Gou-Jen
• Format: Journal Article
• Language: English
• Published: New York IEEE 01.08.2023; The Institute of Electrical and Electronics Engineers, Inc. (IEEE)
• Subjects: Antibodies; electrochemical immunosensor; electrochemical impedance spectroscopy; Electrodes; Erythrocytes; glycosylated hemoglobin detection; Gold; Hemoglobin; Heparin; Immunosensors; monolayer of gold nanoparticles; Monolayers; Nanoparticles; Polyethylene terephthalate; Self-assembled monolayers; Self-assembly; Substrates; Thin films
• ISSN: 1530-437X; 1558-1748
• DOI: 10.1109/JSEN.2023.3289075

This study developed a novel electrochemical glycosylated hemoglobin (HbA1c) immunosensor. Polyethylene terephthalate (PET) was used as a substrate. After gold (Au) film sputtering on the PET surface, 1,6-hexanedithol with thiol groups on both terminals was used as an intermediary to attach a monolayer of uniformly distributed Au nanoparticles (AuNPs) to the Au thin film to form a nanostructured electrode. HbA1c antibodies were successfully immobilized on the AuNP monolayer via the self-assembled monolayer approach. To mimic a real clinical detection environment, a calibration curve for HbA1C detection was performed in a mixture solution containing heparin sodium, red blood cell lysis buffer, artificial human serum and hemoglobin. The experimental results revealed that the proposed HbA1c immunosensor detected HbA1c in a linear range from 2,500 to 10,000 ng/mL with a limit of detection of 2,253 ng/mL. The developed HbA1c-sensing chip offers the advantages of simple operation, low cost, short detection time and provides high feasibility for clinical application.