Preparation of White Adipose Tissue for Transmission Electron Microscopy: An Alternative Technique

An alternative fixation method for preparing white adipose tissue for transmission electron microscopy is described. The procedure uses a primary fixation mixture of glutaraldehyde and acrolein. In our hands, this protocol produces superior ultrastructural morphology and fewer artifacts than traditi...

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Bibliographic Details
Published inJournal of histotechnology Vol. 27; no. 1; pp. 43 - 45
Main Authors Boucherg, G. G., Brees, D. J., Jakowski, A. B.
Format Journal Article
LanguageEnglish
Published Taylor & Francis 01.03.2004
Subjects
adipose tissue
fixation
ultrastructure
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Summary:An alternative fixation method for preparing white adipose tissue for transmission electron microscopy is described. The procedure uses a primary fixation mixture of glutaraldehyde and acrolein. In our hands, this protocol produces superior ultrastructural morphology and fewer artifacts than traditional immersion fixation. This technique can be used in cases where perfusion fixation is neither feasible nor possible. (The J Histotechnol 27:43, 2004) Submitted: August 28, 2003; Accepted: October 31, 2003
ISSN:0147-8885
2046-0236
DOI:10.1179/his.2004.27.1.43