Study on Targeting and in vitro Anti-oxidation of Baicalin Solid Lipid Nanoparticles

• Published in: Chinese herbal medicines Vol. 4; no. 4; pp. 335 - 339
• Main Authors: PING, Yang, YU, Lian, HU, Yan-qiu, MA, Li-na, CAO, Yue-sheng, ZHANG, Lei, MIYAMURA, Mitsuhiko, YOKOTA, Junko, YOSHIOKA, Saburo
• Format: Journal Article
• Language: English
• Published: Elsevier B.V 01.11.2012
• Subjects: activity; anti-oxidative; baicalin; distribution; in vitro anti-oxidative activity; lipid; liver-targeted; nanoparticals; solid; solid lipid nanoparticals; tissue; tissue distribution; vitro; liver-targeted; in vitro anti-oxidative activity; baicalin; solid lipid nanoparticals; tissue distribution
• ISSN: 1674-6384; 2589-3610
• DOI: 10.3969/j.issn.1674-6348.2012.04.012

Objective To prepare liver-targeted baicalin solid lipid nanoparticles(BSLNs) and to study their in vitro anti-oxidative activity.Methods BSLNs were prepared by emulsification ultrasonic dispersion method and characterized by transmission electron microscopy and laser particle size distribution;The tissue in vivo distribution was detected by pharmacokinetics;In vitro anti-superoxide dismutase(SOD) activity and reduction capacity of BSLNs were determined;The ability of removing hydroxyl radical was determined by phenanthroline-Fe2+ oxidation.Results The best prescription was baicalin-soybean lecithin-glyceryl monostearate-poloxamer 188(1:5:15:30);The encapsulation efficiency and drug loading were 84.7% and 5.65%,respectively,mean size of particles was(68.6 ± 8) nm,Zeta potential was 22.13 mV;The in vitro anti-oxidant results showed that BSLNs had a significant inhibitory effect on SOD and a strong reducing capacity as well as a removing hydroxide radical ability.The targeting rate of BSLNs was 6.931 for liver.Conclusion The results demonstrate that BSLNs could enhance the liver targeting ability and in vitro anti-oxidative activity significantly.