Influence of Chronic Lead Intoxication on Functional Characteristics and Isoform Composition of Left Ventricular Myosin in the Rat Heart

The chronic lead intoxication was modelled by injecting outbred rats intraperitoneally with lead acetate 3 times a week for 5 weeks. Using an in vitro motility assay, it was shown that lead intoxication causes changes in in vitro actin–myosin interaction, specifically, a decrease in the maximum slid...

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Bibliographic Details
Published inJournal of evolutionary biochemistry and physiology Vol. 57; no. 4; pp. 896 - 903
Main Authors Gerzen, O. P., Nabiev, S. R., Nikitina, L. V.
Format Journal Article
LanguageEnglish
Published Moscow Pleiades Publishing 01.07.2021
Springer Nature B.V
Subjects
Actin
Adenosine triphosphatase
Animal Physiology
Biochemistry
Biomedical and Life Sciences
Calcium
Evolutionary Biology
Experimental Papers
Filaments
Heart
Intoxication
Isometric
Life Sciences
Myocardium
Myosin
Statistical analysis
Velocity
Ventricle
cardiotoxicity
in vitro motility assay
actin–myosin interaction
lead
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Summary:The chronic lead intoxication was modelled by injecting outbred rats intraperitoneally with lead acetate 3 times a week for 5 weeks. Using an in vitro motility assay, it was shown that lead intoxication causes changes in in vitro actin–myosin interaction, specifically, a decrease in the maximum sliding velocity of native thin filaments over myosin isolated from the left ventricular myocardium of the rat heart. No statistically significant changes were found in the calcium sensitivity and cooperativity of the “ p Ca–velocity” curve, characteristics of the motile filament fraction, and isometric force. Using electrophoretic separation of proteins, a shift in the ratio of myosin heavy chains toward β-chains with a lower ATPase activity was found.
ISSN:0022-0930
1608-3202
DOI:10.1134/S002209302104013X