Technical pitfalls encountered in PCR quantification using microsatellites

Quantitative PCR has proved useful for different purposes, including the detection of particular genetic changes, such as deletions and duplications in several inherited disorders. Using patients with the known duplication mutation for Charcot-Marie-Tooth disease Type 1A as examples, the importance...

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Bibliographic Details
Published inGenetic testing Vol. 1; no. 3; p. 213
Main Authors Qu, Y, Whetsell, L, Carpenter, N J
Format Journal Article
LanguageEnglish
Published United States 01.01.1997
Subjects
Alleles
Case-Control Studies
Charcot-Marie-Tooth Disease - classification
Charcot-Marie-Tooth Disease - genetics
DNA - genetics
DNA - isolation & purification
Electrophoresis
Humans
Microsatellite Repeats
Polymerase Chain Reaction - methods
Polymerase Chain Reaction - statistics & numerical data
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Summary:Quantitative PCR has proved useful for different purposes, including the detection of particular genetic changes, such as deletions and duplications in several inherited disorders. Using patients with the known duplication mutation for Charcot-Marie-Tooth disease Type 1A as examples, the importance of selecting informative microsatellite loci and proper electrophoretic conditions so as to eliminate potential sources of error in quantitative PCR studies is discussed.
ISSN:1090-6576
DOI:10.1089/gte.1997.1.213