Pharmacology of dopamine D3 receptors in the islands of Calleja of the rat using quantitative receptor autoradiography

• Published in: European journal of pharmacology Vol. 261; no. 1-2; pp. 179 - 183
• Main Authors: HILLEFORS-BERGLUND, M, VON EULER, G
• Format: Journal Article
• Language: English
• Published: Amsterdam Elsevier 11.08.1994
• Subjects: Animals; Autoradiography; Biological and medical sciences; Cell Line; Central nervous system; Central neurotransmission. Neuromudulation. Pathways and receptors; Dopamine Agonists - pharmacology; Dopamine Antagonists - pharmacology; Fundamental and applied biological sciences. Psychology; Kinetics; Male; Olfactory Bulb - drug effects; Olfactory Bulb - metabolism; Rats; Rats, Sprague-Dawley; Receptors, Dopamine - drug effects; Receptors, Dopamine D2; Receptors, Dopamine D3; Regression Analysis; Remoxipride - pharmacology; Tetrahydronaphthalenes - pharmacokinetics; Vertebrates: nervous system and sense organs; Autoradiography; Vertebrata; Mammalia; Rat; Olfactory tubercle; Rodentia; Central nervous system; Quantization; Identification; D3 Dopamine receptor; Brain (vertebrata)
• ISSN: 0014-2999; 1879-0712
• DOI: 10.1016/0014-2999(94)90317-4

The pharmacological properties of [3H]7-hydroxy-N,N-di-n-propyl-2- aminotetralin ([3H]7-OH-DPAT) binding sites in the islands of Calleja of the rat were studied using quantitative receptor autoradiography. The KD and the Bmax values of [3H]7-OH-DPAT binding were about 1.6 nM and 100 fmol/mg protein, respectively. The rank order of potency was R(-)-propylnorapomorphine > 7-OH-DPAT approximately haloperidol > raclopride > dopamine > remoxipride. Remoxipride injected in vivo (0.03-100 mumol/kg i.p., 1 h before decapitation) did not inhibit subsequent [3H]7-OH-DPAT binding. These results indicate that the pharmacological profile of dopamine D3 receptors in the islands of Calleja of the rat resembles that obtained from cell lines or membrane preparations.