Dinitrosyl Iron Complexes with Glutathione Ligands Intercept Peroxynitrite and Protect Hemoglobin from Oxidative Modification

• Published in: Applied biochemistry and microbiology Vol. 57; no. 4; pp. 411 - 420
• Main Authors: Kosmachevskaya, O. V., Nasybullina, E. I., Shumaev, K. B., Chumikina, L. V., Arabova, L. I., Yaglova, N. V., Obernikhin, S. S., Topunov, A. F.
• Format: Journal Article
• Language: English
• Published: Moscow Pleiades Publishing 01.07.2021; Springer Nature B.V
• Subjects: Antioxidants; Biochemistry; Biomedical and Life Sciences; Carbonyl compounds; Carbonyls; Coordination compounds; Glutathione; Heme; Hemoglobin; Interceptors; Iron; Life Sciences; Ligands; Medical Microbiology; Microbiology; Nitric oxide; Oxidation; Peroxynitrite; Reagents; Tryptophan; Tyrosine; dinitrosyl iron complexes; peroxynitrite; carbonyl derivatives; oxidative modification; glutathione; hemoglobin
• ISSN: 0003-6838; 1608-3024
• DOI: 10.1134/S0003683821040098

Dinitrosyl iron complexes with glutathione ligands (GS-DNICs) are a physiological form of nitric oxide that is stored and transported in the organism. In addition, these complexes can act as antioxidants and antiradical agents. It was shown that GS-DNICs protect hemoglobin (Hb) from oxidative modification by peroxynitrite. They prevent the formation of carbonyl Hb derivatives, the oxidation of tryptophan and tyrosine residues, degradation of the heme group, and the formation of interprotein cross-links. These complexes also inhibit the oxidation of dihydrorhodamine by peroxynitrite, which forms via the decomposition of 3-morpholinosyndonimine. In some cases, the antioxidant effect of GS-DNICs is comparable to that of reduced glutathione. The results allow GS-DNICs to be considered peroxynitrite interceptors and Hb protectors.