Prostaglandin E2 alters terminal glycosylation of high molecular weight glycoproteins, released by pig gastric mucous cells in vitro

• Published in: Prostaglandins, leukotrienes and essential fatty acids Vol. 52; no. 5; pp. 333 - 340
• Main Authors: ENSS, M.-L, SCHMIDT-WITTIG, U, HEIM, H.-K, SEWING, K.-F
• Format: Journal Article
• Language: English
• Published: Kidlington Elsevier 01.05.1995
• Subjects: Acetylglucosamine - metabolism; Animals; Biological and medical sciences; Cells, Cultured; Chromatography, Gel; Dinoprostone - pharmacology; Enzyme-Linked Immunosorbent Assay; Fundamental and applied biological sciences. Psychology; Gastric Mucosa - metabolism; Glycoproteins - metabolism; Glycosylation; Lectins; Molecular Weight; Oligosaccharides - metabolism; Periodic Acid-Schiff Reaction; Stomach; Swine; Vertebrates: digestive system; Stomach; Digestive system; Arachidonic acid derivatives; Prostaglandin E2; Glycoproteins; Glycosylation; Mucous cell; Pig; Vertebrata; Mammalia; Eicosanoid; Artiodactyla; Ungulata
• ISSN: 0952-3278; 1532-2823
• DOI: 10.1016/0952-3278(95)90035-7

The gastric mucus layer consists of high molecular weight glycoproteins (HMG). E-Type prostaglandins (PGs) stimulate total HMG release from isolated gastric mucous cells. We determined the effects of PGE2 on HMG glycosylation. Pig gastric mucous cells were cultured for 20 h with 1 mumol/l PGE2. Released HMG were isolated by gel chromatography and periodic acid-Schiff (PAS)-positive sugars and protein-bound [14C]GlcNAc were determined. Monosaccharides terminally linked to HMG oligosaccharide chains were monitored by lectin enzyme linked immunosorbent assay (ELISA): N-acetylglucosamine (GlcNAc) with Datura stramonium agglutinin, N-acetylgalactosamine (GalNAc) with soy bean agglutinin, fucose (Fuc) with Ulex europaeus I agglutinin and sialic acids (Sial) with Sambucus nigra agglutinin. PGE2 stimulated total HMG release, indicated by an increase of PAS-positive sugars to 170% and [14C]GlcNAc to 220% of controls. Terminal GlcNAc increased to 128%, GalNAc to 133%, Fuc to 165% and Sial to 182%. In addition to stimulation of total HMG release, PGE2 caused alterations of HMG glycosylation, which may modulate HMG viscosity and microbiological barrier function.