Effect of extracellular calcium concentration on potency of muscarinic agonists at M1 and M2 receptors in rabbit vas deferens

The M1 potency (decrease in neurogenic response) of carbachol, oxotremorine, muscarine, arecaidine propargly ester (APE) and 4-(4-chlorophenylcarbamoyloxy)-2-butynyltrimethylammonium iodide (4-Cl-McN-A-343) in rabbit field-stimulated vas deferens (prostatic segments) increased up to 15-fold when cal...

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Bibliographic Details
Published inEuropean journal of pharmacology Vol. 203; no. 3; p. 417
Main Authors Dörje, F, Rettenmayr, N M, Mutschler, E, Lambrecht, G
Format Journal Article
LanguageEnglish
Published Netherlands 22.10.1991
Subjects
(4-(m-Chlorophenylcarbamoyloxy)-2-butynyl)trimethylammonium Chloride - pharmacology
Animals
Calcium - metabolism
Electric Stimulation
In Vitro Techniques
Male
Muscle, Smooth - drug effects
Parasympathomimetics - pharmacology
Pirenzepine - pharmacology
Rabbits
Receptors, Muscarinic - drug effects
Vas Deferens - drug effects
Vas Deferens - physiology
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Summary:The M1 potency (decrease in neurogenic response) of carbachol, oxotremorine, muscarine, arecaidine propargly ester (APE) and 4-(4-chlorophenylcarbamoyloxy)-2-butynyltrimethylammonium iodide (4-Cl-McN-A-343) in rabbit field-stimulated vas deferens (prostatic segments) increased up to 15-fold when calcium was lowered (3.5-1.0 nM). The M2 receptor-mediated increase in twitch height in response to carbachol and the M1 affinity of pirenzepine were independent of calcium concentration (2.5 and 1.0 mM). Thus, prostatic segments of rabbit vas deferens can be used successfully at 1.0 mM calcium to determine M1 potencies of agonists and M1 affinities of antagonists without counteracting stimulation of M2 receptors.
ISSN:0014-2999
DOI:10.1016/0014-2999(91)90900-B