Aldehydes from metal ion- and lipoxygenase-induced lipid peroxidation : detection by 1H-n.m.r. spectroscopy

The modification of lipoproteins by reactive aldehydes formed via lipid peroxidation is thought to be a key process in the pathogenesis of atherosclerosis. We show that 1H-n.m.r. spectroscopy can readily be used to detect a variety of different aldehydes resulting from peroxidation of liposomes indu...

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Bibliographic Details
Published inBiochemical journal Vol. 289; no. 1; pp. 149 - 153
Main Authors LODGE, J. K, PATEL, S. U, SADLER, P. J
Format Journal Article
LanguageEnglish
Published Colchester Portland Press 1993
Subjects
Aldehydes - analysis
Biological and medical sciences
Copper - pharmacology
Diverse techniques
Fundamental and applied biological sciences. Psychology
Lipid Peroxidation
Lipoproteins, LDL - chemistry
Liposomes
Lipoxygenase - pharmacology
Magnetic Resonance Spectroscopy
Molecular and cellular biology
Protons
Enzyme
Metal ion
Lipids
NMR spectrometry
Lipoprotein
Method
Aldehyde
Detection
Lipoxygenase
Peroxidation
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Summary:The modification of lipoproteins by reactive aldehydes formed via lipid peroxidation is thought to be a key process in the pathogenesis of atherosclerosis. We show that 1H-n.m.r. spectroscopy can readily be used to detect a variety of different aldehydes resulting from peroxidation of liposomes induced by Fenton's reagent or lipoxygenase, and aldehydes arising from copper-induced reactions of low-density lipoprotein. There is a clear contrast between the major aldehydic products arising from metal-ion- and lipoxygenase-induced reactions.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
ISSN:0264-6021
1470-8728
DOI:10.1042/bj2890149