Optimization of Extraction of Fpase from the Fermented Bran of Aspergillus niger in Solid State Fermentation

A local isolate of Aspergillus niger was cultivated under optimal growth conditions on wheat bran in solid state fermentation. Filter paperase from fermented bran was separately extracted with different solvents to test the recovery of the enzyme. Among solvents tested, distilled water served as the...

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Published inJournal of applied biological chemistry Vol. 51; no. 4
Main Authors Subhosh Chandra, M. (Sri Krishnadevaraya University, Anantapur, India), Rajasekhar Reddy, B. (Sri Krishnadevaraya University, Anantapur, India), Choi, Y.L. (Dong-A University, Busan, Republic of Korea), E-mail: ylchoi@dau.ac.kr
Format Journal Article
LanguageEnglish
Published 01.08.2008
Subjects
ASPERGILLUS NIGER
EXTRACCION
EXTRACTION
Fpase
solid state fermentation
wheat bran
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Summary:A local isolate of Aspergillus niger was cultivated under optimal growth conditions on wheat bran in solid state fermentation. Filter paperase from fermented bran was separately extracted with different solvents to test the recovery of the enzyme. Among solvents tested, distilled water served as the best leachate, thus the conditions were further optimized with distilled water. After two washes of fermented bran with distilled water for 1.5 h each under stationary conditions at 1 g wheat bran: 5 mL distilled water, the maximum recovery of 13.5 U g-¹ of wheat bran was obtained.
Bibliography:F60
2009002474
ISSN:1976-0442
DOI:10.3839/jabc.2008.028