A Potential Differentiation of Adipose and Hair Follicle-derived Mesenchymal Stem Cells to Generate Neurons Induced with EGF, FGF, PDGF and Forskolin

• Published in: Research journal of pharmacy and technology Vol. 13; no. 1; pp. 275 - 281
• Main Authors: Rantam, Fedik Abdul, Nugraha, Alexander Patera, Ferdiansyah, Ferdiansyah, Purwati, Purwati, Bumi, Candra, Susilowati, Helen, Hendrianto, Eryk, Utomo, Dwikora Novembri, Suroto, Heri, Sumartono, Christijogo, Setiawati, Rosy, Prakoeswa, Cita Rosita, Indramaya, Diah Mira
• Format: Journal Article
• Language: English
• Published: Raipur A&V Publications 2020
• Subjects: Epidermal growth factor; Fibroblasts; Hair; Longitudinal studies; Medical research; Neurons; Stem cells; East Java Indonesia; United States > US; Indonesia
• ISSN: 0974-3618; 0974-360X; 0974-306X
• DOI: 10.5958/0974-360X.2020.00056.6

[...]the use of MSCs from older donors is lower than younger donors. Additionally, hADMSCs have higher and greater multipotent rates in culture compared with Bone Marrow Mesenchymal Stem Cells (BMSCs)7. [...]hADMSCs have been found not only to differentiate into adipocytes but also to into various other cell type such as myocytes, osteoblasts, chondrocytes, and neuronal cells8,9. Monolayer cells were detached using trypsin (0.025%); next, after washing and counting counted using cell counter (Becton Dickson, USA) approximately 5000 cells were dropped in a volume of 10 pl PBS into a COOKE® well and incubated for 1 hour at 37°C. After fixing with acetone, washing in - 20°C PBS with 1% serum, and reacting with monoclonal antibody anti human, anti-CD105 (Becton Dickson, US, Cat no 563264), anti-CD90 (Becton Dickson, US, Cat no 5662385) and anti-CD45 antibodies anti human with concentration 1:200 (Becton Dickson, US, Cat no 340910), the cells were analyzed under a fluorescence microscope (BX63, Olympus, US).9,12,17 Additionally, FACS Calibur flowcytometry (Becton Dickson, USA) were used to identify the CD90 and CD45 phenotypic marker proteins on the superficial membranes.18 Chemical Induction of Adipose Derived Mesenchymal Stem Cells and Hair Follicle Derived Mesenchymal Stem Cells: ADMSCs and HFDMSCs were also chemically induced to become neuron-like cells with serum-free DMEM/F12 (with high glucose; Gibco/BRL) containing one of the following: [...]the MSCs were examined for the neuron-like cell markers with monoclonal antibody antihuman GFAP (cat no.