Expression and Correlation Research of MicroRNA10a-5p and PIK3CA in Middle Ear Cholesteatoma

• Published in: Journal of International Advanced Otology Vol. 19; no. 3; pp. 212 - 216
• Main Authors: Yang, Jing, Yan, Wei, Tang, Susu, Huang, Zhikun, Ye, Mingyuan, Lu, Zheng, Liu, Qianxu
• Format: Journal Article
• Language: English
• Published: Turkey AVES 01.06.2023
• Subjects: Cell Proliferation - genetics; Cholesteatoma, Middle Ear - genetics; Class I Phosphatidylinositol 3-Kinases - genetics; Class I Phosphatidylinositol 3-Kinases - metabolism; Humans; MicroRNAs - genetics
• ISSN: 2148-3817; 2148-3817
• DOI: 10.5152/iao.2023.22863

BACKGROUND: This study aimed to examine the roles of miR-10a-5p and phosphatidylinositol-4,5-bisphosphonate 3-kinase catalytic subunit α in the pathogenesis of middle ear cholesteatoma. METHODS: We enrolled 27 patients with middle ear cholesteatoma and collected samples of intraoperative cholesteatoma and normal posterior ear skin tissues. The mRNA expression levels of miR-10a-5p and PIK3CA were detected using real-time quantitative polymerase chain reaction. PIK3CA protein expression was measured by immunohistochemistry and western blotting. RESULTS: Middle ear cholesteatoma tissues showed significantly lower miR-10a-5p expression levels and significantly higher PIK3CA expression levels than normal posterior ear skin tissues (both P < .05). Furthermore, the miR-10a-5p and PIK3CA expression levels were significantly negatively correlated in middle ear cholesteatoma tissues (r = −0.926, P < .001). CONCLUSION: Low miR-10a-5p expression levels in middle ear cholesteatoma tissues may inhibit the growth and proliferation of cholesteatoma, whereas high PIK3CA expression level may promote its growth and proliferation. In addition, miR-10a-5p may affect the proliferation and differentiation of cholesteatoma by negatively regulating its target gene, PIK3CA. Cite this article as: Yang J, Yan W, Tang S, et al. Expression and correlation research of MicroRNA-10a-5p and PIK3CA in middle ear cholesteatoma. J Int Adv Otol. 2023;19(3):212-216.