Fraud-proof methylcellulose-based fish freshness indicator: Reversibility in halochromic sensing of basic volatiles is tailored by ionic strength

• Published in: International journal of biological macromolecules Vol. 277; no. Pt 4; p. 134486
• Main Authors: Silva, R.R.A., de Freitas, P.A.V., de Oliveira, T.V., Teixeira, S.C., Rigolon, T.C.B., Stringheta, P.C., Otoni, C.G., Soares, N. de F.F.
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 01.10.2024
• Subjects: Animals; Anthocyanins; Anthocyanins - analysis; Anthocyanins - chemistry; Color; Colorimetric sensors; Colorimetry - methods; Fish freshness; Fishes; Food Packaging - methods; Food safety; Ionic strength; Methylcellulose - analogs & derivatives; Methylcellulose - chemistry; Osmolar Concentration; Smart packaging; Volatile Organic Compounds - analysis; Volatile Organic Compounds - chemistry; Colorimetric sensors; Anthocyanins; Food safety; Ionic strength; Fish freshness; Smart packaging
• ISSN: 0141-8130; 1879-0003; 1879-0003
• DOI: 10.1016/j.ijbiomac.2024.134486

Food from animal sources (e.g., fish) represents the food group most likely to disseminate diseases to humans. To prevent food contamination and foodborne illnesses, intelligent packaging has been developed to monitor fish freshness by real-time tracking their physicochemical attributes and informing consumers about their conservation state. In this context, we investigated the influence of ionic strength (IS) provided by CaCl2 on the chromatic response of anthocyanin açai extracts incorporated into methylcellulose (MC) within hydrocolloid-based colorimetric sensors for monitoring the freshness of Lambari fish. The color sensitivity of the sensors was modulated by IS in the presence of NH3 volatile and/or TVB-N. Increasing IS led to a plasticizing effect in the MC matrix, which influenced the chromatic properties of anthocyanin in the presence of NH3 and/or TVB-N. The perception of distinct colors by untrained eyes improved from 10 min with the control sensor to 2.5 min for sensors with IS >50 mM. Adjusting the IS to 500 mM with LiCl, CaCl2, or MgCl2 resulted in gray-green, blue, or moss-green colors, respectively, diverging from the control sensor's color (pink and gray) after 10 min of ammonia exposure, confirming salt-induced copigmentation. Color irreversibility in the sensors was achieved when the IS exceeded 250 mM. Through principal component analysis, we statistically validate the efficacy of the sensor in assessing the freshness of Lambari fish. The sensor maintained its color-change capability even after 60 d of storage and was able to classify Lambari fish freshness according to Brazilian and European standards. This study elucidates the interrelation between the structures and properties of natural compounds such as MC, anthocyanin, and CaCl2, providing a method to control the chromatic properties of sensors intended to monitor food quality, safety, and shelf-life. [Display omitted] •Methylcellulose-based sensors were developed to monitor Lambari fish freshness levels.•Ionic strength modulated the detection sensitivity of NH3 and TVB-N.•Copigments in hydrocolloid polymer sensors stabilized and broadened color spectrum.•Sensors exhibited irreversible color change above 250 mM ionic strength.•TVB-N and pH levels in fish were predicted via smartphone photographs.