Comprehensive two-dimensional countercurrent chromatography × gas chromatography characterization of Artemisia argyi essential oil

• Published in: Analytica chimica acta Vol. 1237; p. 340614
• Main Authors: Wen, Weiyi, Xu, Ping, Xiang, Haiping, Wen, Mengyi, Ye, Xuemin, Chu, Chu, Tong, Shengqiang
• Format: Journal Article
• Language: English
• Published: Elsevier B.V 02.01.2023
• Subjects: Comprehensive two-dimensional chromatography; Contour plot map; Countercurrent chromatography; Gas chromatography; Orthogonality; Gas chromatography; Countercurrent chromatography; CCC; 2D; 1D; Contour plot map; Orthogonality; GC; Comprehensive two-dimensional chromatography
• ISSN: 0003-2670; 1873-4324
• DOI: 10.1016/j.aca.2022.340614

A comprehensive two-dimensional (2D) countercurrent chromatography (CCC) × gas chromatography (GC) was investigated for characterization of chemical constituents of Artemisia argyi essential oil, and orthogonality for the 2D chromatographic system was evaluated. A solvent system composed of n-hexane/acetonitrile/methanol (2:2:1, v/v/v) was selected for first dimensional separation of Artemisia argyi essential oil. Then all CCC fractions were analyzed by GC, which provided a wealth of information regarding the composition of the essential oil. Visualization of chemical compositions obtained from the comprehensive 2D CCC × GC separation was achieved by creation of a 2D contour plot map. Total peak capacity was evaluated and approximately 1392 peaks were obtained through a comprehensive 2D CCC × GC separation. A high spatial coverage and a low linear correlation coefficient were achieved. Meanwhile, all compounds were identified by GC-MS. The obtained 2D contour plot could be divided into six zones to show the characteristic chemical compositions. Six zones could be divided into different component groups, including monoterpenes, sesquiterpenes, monoterpene alcohols, phenols, aldehydes, ketones and esters, which could be used to identify compounds that have not been reported, and to predict the structure of unknown compounds in Artemisia argyi essential oil and comprehensively characterize fingerprint peak. [Display omitted] •Comprehensive 2D CCC × GC chromatographic analysis of Artemisia argyi essential oil.•Orthogonality metric was evaluated by convex hull and linear correlation coefficient.•Development of a 2D contour map for a complete and informative assessment of sample.•Separation and characterization of new constituents in Artemisia argyi essential oil.•Zonal substance classification for comprehensive identification of fingerprint peaks.