Determination of tetracycline residues in potatoes and soil by LC-MS/MS: Method development, validation, and risk assessment

• Published in: Food chemistry Vol. 461; p. 140841
• Main Authors: Khaled, Omar, Ryad, Lamia, Eissa, Fawzy
• Format: Journal Article
• Language: English
• Published: England Elsevier Ltd 15.12.2024
• Subjects: Anti-Bacterial Agents - analysis; Chromatography, High Pressure Liquid; Chromatography, Liquid - methods; Food Contamination - analysis; Liquid Chromatography-Mass Spectrometry; Method development; Method validation; Potato uptake; Risk Assessment; Soil - chemistry; Soil Pollutants - analysis; Soil pollution; Solanum tuberosum - chemistry; Tandem Mass Spectrometry - methods; Tetracycline residues; Tetracyclines - analysis; THQ; Potato uptake; Method development; Method validation; Soil pollution; THQ; Tetracycline residues
• ISSN: 0308-8146; 1873-7072; 1873-7072
• DOI: 10.1016/j.foodchem.2024.140841

A method utilizing liquid-liquid extraction (LLE) coupled with liquid chromatography-tandem mass spectrometry (LC-MS/MS) was developed and validated according to the Commission Implementing Regulation (CIR) EU 2021/808 for quantifying four tetracyclines (TCs) in potatoes and soil. The method demonstrated recovery values ranging from 70 to 121% and precision (repeatability and within-laboratory reproducibility), with coefficient of variation (CV) values below 18% for all TCs in both matrices. The limits of quantification (LOQs) for the TCs ranged from 0.90 to 1.87 μg/kg in potatoes and from 0.68 to 1.25 μg/kg in soil. The decision limit (CCα) and detection capability (CCβ) ranged from 10.4 to 12.3 μg/kg and 11.9 to 14.3 μg/kg, respectively. Analysis of 538 potato and soil samples from Egyptian farms revealed a 13.2% occurrence of TC residues, with a higher frequency in soil (19.33%) than in potatoes (7.06%). Target hazard quotient (THQ) values indicated that TC residues in potatoes do not pose a health risk to Egyptian consumers. [Display omitted] •A method for quantifying tetracycline residues in potatoes and soil was developed.•Recovery values ranged from 70 to 121%, while precision, as CV values, were all below 18%.•Tetracycline (TC) residues were detected in 7.06% of potato samples (n = 269).•19.33% of soil samples (n = 269) from Egyptian potato farms had TC residues.•TC residues in potatoes do not pose a health risk to consumers based on THQ values.