Optimizing tomato waste hydrolysate for enhanced fucoxanthin biosynthesis in mixotrophic cultivation of Isochrysis galbana

• Published in: Bioresource technology Vol. 413; p. 131453
• Main Authors: Fan, Xi-Wen, Sun, Han, Ayittey, Derek M., Zhou, Zhi-gang, Sze Ki Lin, Carol, Tang, Tao, Sun, Zheng
• Format: Journal Article
• Language: English
• Published: England Elsevier Ltd 01.12.2024
• Subjects: Algal Bioproducts; Carotenoid biosynthesis; Lycopene; Waste valorization; β-carotene; Carotenoid biosynthesis; Waste valorization; β-carotene; Lycopene; Algal Bioproducts
• ISSN: 0960-8524; 1873-2976; 1873-2976
• DOI: 10.1016/j.biortech.2024.131453

[Display omitted] •Tomato waste enhances I. galbana cultivation efficiency for producing fucoxanthin.•Soluble lycopene and β-carotene upregulated genes expressions PSY, LCYB, and ZEP3.•Hydrolyzed tomato waste in growth medium enhances fucoxanthin synthesis.•Tomato waste as nutrient source sustainably yields valuable products in microalgae. Vegetable waste, rich in bioactive compounds, offers a promising resource for producing value-added products. This study explored the use of tomato waste, containing glucose (40 mg/g), lycopene (95.12 μg/g), and β-carotene (24.31 μg/g), for cultivating fucoxanthin-rich Isochrysis galbana. Water-soluble lycopene (2.0 μg/mL) and β-carotene (0.4 μg/mL) effectively upregulated key carotenoid synthesis genes and boosted cell growth and fucoxanthin production (3.64 and 3.60 pg/cell, respectively) within 10 days in a mixotrophic culture. Optimized tomato waste hydrolysate achieved a high cell density of 1.21 × 107 cells/mL, 2.13 g/L biomass, and 21.02 mg/g fucoxanthin. This study highlights the potential of combining tomato waste with microalgae for a novel and innovative approach towards waste management and resource utilization.