Antibacterial mechanism of metabolites of Lactobacillus plantarum against Pseudomonas lundensis and their application in dry-aged beef preservation

• Published in: Food chemistry Vol. 460; no. Pt 1; p. 140463
• Main Authors: Rao, Wei, Wu, Jinchong, Fang, Ziying, Chen, Zhaomin, Wu, Jianfeng, Fang, Xiang
• Format: Journal Article
• Language: English
• Published: England Elsevier Ltd 01.12.2024
• Subjects: Animals; Anti-Bacterial Agents - chemistry; Anti-Bacterial Agents - pharmacology; Antibacterial mechanism; Biofilms - drug effects; Cattle; Dry-aged beef; Food Preservation - methods; Lactobacillus plantarum; Lactobacillus plantarum - chemistry; Lactobacillus plantarum - growth & development; Lactobacillus plantarum - metabolism; Metabolites; Pseudomonas - growth & development; Pseudomonas - metabolism; Pseudomonas lundensis; Red Meat - analysis; Red Meat - microbiology; Dry-aged beef; Lactobacillus plantarum; Metabolites; Pseudomonas lundensis; Antibacterial mechanism
• ISSN: 0308-8146; 1873-7072; 1873-7072
• DOI: 10.1016/j.foodchem.2024.140463

In this study, the antibacterial mechanism of metabolites of Lactobacillus plantarum SCB2505 (MLp SCB2505) against Pseudomonas lundensis (P. lundensis) SCB2605 was investigated, along with evaluation of their preservative effects on dry-aged beef. The results demonstrated the effective inhibition of MLp SCB2505 on the growth and biofilm synthesis of P. lundensis. The treatment with MLp SCB2505 led to the compromised membrane integrity, as evidenced by reduced intracellular ATP content, increased extracellular AKPase, K+ and protein content, as well as disrupted cell morphology. Further metabolomics analysis revealed that MLp SCB2505 interfered amino acid metabolism, nucleotide metabolism, cofactor and vitamin metabolism, lipid metabolism and respiratory chain in P. lundensis, ultimately leading to the interrupted life activities and even death of the bacteria. Besides, MLp SCB2505 could effectively inhibit the growth of Pseudomonas in dry-aged beef and delay spoilage. These findings propose the potential application of MLp SCB2505 as an antibacterial agent in meat products. [Display omitted] •MLp SCB2505 showed antibacterial effects against P. lundensis•MLp SCB2505 induced morphological changes in P. lundensis•Untargeted metabolomics analysis revealed changes in metabolites in P. lundensis.•A total of 16 metabolic pathways were disturbed after MLp SCB2505 treatment•MLp SCB2505 inhibited the growth of P. lundensis on the surface of dry-aged beef