Gα q/11 signaling tonically modulates nociceptor function and contributes to activity-dependent sensitization

• Published in: Pain (Amsterdam) Vol. 153; no. 1; pp. 184 - 196
• Main Authors: Tappe-Theodor, Anke, Constantin, Cristina E., Tegeder, Irmgard, Lechner, Stefan G., Langeslag, Michiel, Lepcynzsky, Peter, Wirotanseng, Richard I., Kurejova, Martina, Agarwal, Nitin, Nagy, Gergely, Todd, Andrew, Wettschureck, Nina, Offermanns, Stefan, Kress, Michaela, Lewin, Gary R., Kuner, Rohini
• Format: Journal Article
• Language: English
• Published: Philadelphia, PA Elsevier B.V 2012; Elsevier
• Subjects: Biological and medical sciences; Cranial nerves. Spinal roots. Peripheral nerves. Autonomic nervous system. Gustation. Olfaction; Fundamental and applied biological sciences. Psychology; G protein-coupled receptor; Knock-out mice; Medical sciences; Nervous system (semeiology, syndromes); Neurology; Nociceptor; Pain; Somesthesis and somesthetic pathways (proprioception, exteroception, nociception); interoception; electrolocation. Sensory receptors; TTX-resistant sodium channels; Vertebrates: nervous system and sense organs; G protein-coupled receptor; Nociceptor; Pain; Knock-out mice; TTX-resistant sodium channels; Protein kinase C; Calcium; Enzyme; Transferases; Rodentia; Signal transduction; Vertebrata; Mammalia; Mouse; Modulation; Sensitization; Coupling; Intracellular; Release; G protein; Biological receptor
• ISSN: 0304-3959; 1872-6623
• DOI: 10.1016/j.pain.2011.10.014

The functional role of G q/11 G proteins in nociceptors not only spans pathological pain, but, surprisingly, also includes tonic modulation of nociception. Peripheral injury or inflammation leads to a release of mediators capable of binding to a variety of ion channels and receptors. Among these are the 7-transmembrane receptors (G protein-coupled receptors) coupling to G s, G i/o, G 12/13, or G q/11 G proteins. Each of the G protein-coupled receptor pathways is involved in nociceptive modulation and pain processing, but the relative contribution of individual signaling pathways in vivo has not yet been worked out. The G q/G 11 signaling branch is of particular interest because it leads to the activation of phospholipase C-β, protein kinase C, the release of calcium from intracellular stores, and it modulates extracellular regulated kinases. To investigate the contribution of the entire G q/11-signaling pathway in nociceptors towards regulation of pain, we generated double-deficient mice lacking G q/11 selectively in nociceptors using a conditional gene-targeting approach. We observed that nociceptor-specific loss of G q and G 11 results in reduced pain hypersensitivity following paw inflammation or spared nerve injury. Surprisingly, our behavioral and electrophysiological experiments also indicated defects in basal mechanical sensitivity in G q/11 mutant mice, suggesting a novel function for G q/11 in tonic modulation of acute nociception. Patch-clamp recordings revealed changes in voltage-dependent tetrodotoxin-resistant and tetrodotoxin-sensitive sodium channels in nociceptors upon a loss of G q/11, whereas potassium currents remained unchanged. Our results indicate that the functional role of the G q/G 11 branch of G-protein signaling in nociceptors in vivo not only spans sensitization mechanisms in pathological pain states, but is also operational in tonic modulation of basal nociception and acute pain.