Dipeptidyl peptidase‐IV inhibitory potential of alpha‐lactalbumin extracted from milk of Gir cows: A Bos indicus species

An attempt has been made to assess the dipeptidyl peptidase‐IV (DPP‐IV) inhibitory potential of α‐lactalbumin separated from milk of Gir cow. α‐lactalbumin hydrolysates were prepared using three different enzymes at different enzyme‐to‐substrate (E:S) ratios (1:25, 1:50 and 1:100) treated for 2‐ to...

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Bibliographic Details
Published inInternational journal of dairy technology Vol. 75; no. 3; pp. 527 - 537
Main Authors Kumar, Ashok, Kumar M H, Sathish, C S, Rajani, Sabikhi, Latha, Naik N, Laxmana
Format Journal Article
LanguageEnglish
Published Oxford Blackwell Publishing Ltd 01.08.2022
Subjects
Bioactive peptides
Bos taurus indicus
Cow's milk
Degree of hydrolysis
DPP‐IV inhibition
High-performance liquid chromatography
Hydrolysates
IC50 value
Lactalbumin
Liquid chromatography
Milk
Pepsin
Peptidase
Peptidases
Substrates
α‐lactalbumin
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Summary:An attempt has been made to assess the dipeptidyl peptidase‐IV (DPP‐IV) inhibitory potential of α‐lactalbumin separated from milk of Gir cow. α‐lactalbumin hydrolysates were prepared using three different enzymes at different enzyme‐to‐substrate (E:S) ratios (1:25, 1:50 and 1:100) treated for 2‐ to 12‐h durations. Pepsin‐treated hydrolysates showed maximum DPP‐IV inhibition of 87.81 ± 0.84% (IC50 = 0.78 mg/mL). The permeate of 3 kDa, which exhibited 84.47 ± 2.29% DPP‐IV inhibition, was fractionated through RP‐HPLC. All fractions were analysed for DPP‐IV inhibition activity. One novel peptide was identified through LC‐MS/MS, which showed adequate DPP‐IV inhibition (IC50 = 4.93 mM). Isolation DPP‐IV inhibitory peptides from milk.
ISSN:1364-727X
1471-0307
DOI:10.1111/1471-0307.12868