Regulation of Methionine Biosynthesis in Escherichia coli: Mapping of the metJ Locus and Properties of a metJ+/metJ-Diploid

MetJ-mutants of Escherichia coli have elevated nonrepressible levels of the enzymes of methionine biosynthesis and S-adenosylmethionine synthetase (ATP:L-methionine S-adenosyltransferase, EC 2.5.1.6). In E. coli, as in Salmonella typhimurium, the metJ locus is close to metB (95% cotransduction of me...

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Bibliographic Details
Published inProceedings of the National Academy of Sciences - PNAS Vol. 68; no. 2; pp. 367 - 371
Main Authors Su, Ching-Hsiang, Greene, Ronald C.
Format Journal Article
LanguageEnglish
Published National Academy of Sciences of the United States of America 01.02.1971
National Acad Sciences
Subjects
Acridines
Bacteriophages
Biological Sciences: Genetics
Biosynthesis
Crossovers
Diploidy
Enzymes
Escherichia coli
Genetic loci
Government regulation
Phenotypes
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Summary:MetJ-mutants of Escherichia coli have elevated nonrepressible levels of the enzymes of methionine biosynthesis and S-adenosylmethionine synthetase (ATP:L-methionine S-adenosyltransferase, EC 2.5.1.6). In E. coli, as in Salmonella typhimurium, the metJ locus is close to metB (95% cotransduction of metB and metJ markers), but in E. coli the order is reversed, with metJ mapping clockwise to metB. A stable merodiploid, heterozygous for metJ, is subject to repression by methionine. Thus, metJ functions via a diffusible product. MetJ could either be a regulatory locus or could code for an enzyme required for the synthesis of a methionine metabolite that functions in the control system.
ISSN:0027-8424
1091-6490
DOI:10.1073/pnas.68.2.367