On‐line hydrogenation in GC‐MS analysis of cyclic fatty acid monomers isolated from heated linseed oil

• Published in: Lipids Vol. 24; no. 4; pp. 347 - 350
• Main Authors: Le Quéré, J. L., Sémon, E., Lanher, B., Sébédio, J. L.
• Format: Journal Article Conference Proceeding
• Language: English
• Published: Berlin/Heidelberg Springer‐Verlag 01.04.1989; Springer
• Subjects: Analytical, structural and metabolic biochemistry; Biological and medical sciences; Fat industries; Food industries; Fundamental and applied biological sciences. Psychology; Lipids; Other biological molecules; Lipids; Identification; Oilseed; Coupled method; Linseed oil; Fatty acids; Hydrogenation; Fiber crop; Oil plant(vegetal); Gas chromatography; Linum usitatissimum; Linaceae; Dicotyledones; Angiospermae; Spermatophyta; Mass spectrometry; Structural analysis
• ISSN: 0024-4201; 1558-9307
• DOI: 10.1007/BF02535175

A simple on‐line hydrogenation method in GC‐MS analysis of unsaturated fatty acid esters is described. Using hydrogen as carrier gas, hydrogenation takes place in a capillary reactor connected to the outlet of the analytical column in the oven of the gas chromatograph. The reactor is a fused silica tube (60 cm×0.32 mm i.d.) coated with palladium acetylacetonate. Selective hydrogenation of olefinic bonds is achieved after a normal chromatographic run. Structural information (carbon‐skeleton, double bond equivalents) can thus be deduced, and structural correlations between the saturated and unsaturated components can be obtained. Structures of cyclic fatty acid esters isolated from heated linseed oil were elucidated using this simple method which was found very useful for structural investigations on unsaturated compounds by GC‐MS.