The effect of an antiendotoxin monoclonal antibody in a rat sepsis model

• Published in: Ensho Vol. 15; no. 4; pp. 323 - 328
• Main Authors: Kimura, Yoshihiko, Abo, Shichisaburo, Kitamura, Michihiko, Izumi, Keiichi, Shikama, Toshio, Minamiya, Yoshihiro, Saito, Satoshi
• Format: Journal Article
• Language: Japanese; English
• Published: The Japanese Society of Inflammation and Regeneration 1995
• Subjects: E5; endotoxin; hydrogen peroxide; pulmonary microcirculation
• ISSN: 0389-4290; 1884-4006
• DOI: 10.2492/jsir1981.15.323

We evaluated the inhibitory effect of pretreatment of a mouse antiendotoxin monoclonal antibody (E5) on hypotension and oxygen radical production in the intact pulmonary microcirculation, using our original method which allows us to visualize the hydrogen peroxide production of white blood cells in vivo. The rat sepsis model was developed by continuous infusion of endotoxin (5 mg/kg/hour) . Blood pressure was recorded by left femoral arterial intubation. After 2 hours, we visualized the hydrogen peroxide production by intravenous administration of 2', 7'-dichlorofluorescin diacetate, and observed the pulmonary microcirculation, using a fluorescence microscope, a SIT TV camera, and a videotape recorder. We calculated the hydrogen peroxide production by image analysis after the experiment. E5 (6 mg/kg), which was injected 1 hour before, significantly prevented the hypotension induced by the endotoxin. We found that the amount of fluorescence significantly decreased in the E5 pretreatment group compared with the endotoxin group. In conclusion, E5 pretreatment inhibits hypotension and hydrogen peroxide production in the intact pulmonary microcirculation associated with endotoxin in a rat sepsis model.