Comprehensive in silico analysis for identification of novel candidate target genes, including DHX36, OPA1, and SENP2, located on chromosome 3q in head and neck cancers

• Published in: Head & neck Vol. 43; no. 1; pp. 288 - 302
• Main Authors: Karatas, Omer Faruk, Capik, Ozel, Barlak, Neslisah, Aydin Karatas, Elanur
• Format: Journal Article
• Language: English
• Published: Hoboken, USA John Wiley & Sons, Inc 01.01.2021; Wiley Subscription Services, Inc
• Subjects: Angiogenesis; Carcinogenesis; Chromosome 3; DHX26; Gene regulation; Head & neck cancer; head and neck cancer; in silico analysis; OPA1; Post-transcription; SENP2; Squamous cell carcinoma; Tumors
• ISSN: 1043-3074; 1097-0347
• DOI: 10.1002/hed.26493

Background Major milestones of head and neck carcinogenesis have been associated with various genetic abnormalities; however, a clear picture of the molecular networks deregulated during the carcinogenesis of head and neck squamous cell carcinoma (HNSC) has not yet completely revealed. Methods In this study, we used in silico tools and online data sets to evaluate the underlying reasons for the expressional changes of genes residing within the chromosome 3q and to help understanding their contributions to HNSC carcinogenesis. Results We found that 13 of 20 most upregulated genes in HNSC are localized to 3q. Further analysis revealed a gene signature consisting of DHX36, OPA1, and SENP2, which showed significant correlation in HNSC samples and potentially be deregulated through similar mechanisms including DNA amplification, transcriptional, and posttranscriptional regulation. Conclusions Considering our findings, we suggest DHX36, OPA1, and SENP2 genes as overexpressed in HNSC tumors and that might be concurrently involved in HNSC carcinogenesis, tumor progression, and induction of angiogenic pathways.