Factors affecting Agrobacterium tumefaciens-mediated gusA expression and opine synthesis in Gladiolus

• Published in: Plant cell reports Vol. 16; no. 6; pp. 389 - 392
• Main Author: Kamo, K
• Format: Journal Article
• Language: English
• Published: Berlin Springer 01.03.1997
• Subjects: Agrobacterium tumefaciens; alkaloids; beta-glucuronidase; Biological and medical sciences; biosynthesis; Biotechnology; cultivars; enzyme activity; etiolation; Fundamental and applied biological sciences. Psychology; gene expression; gene transfer; genes; Genetic engineering; Genetic technics; genetic transformation; genetic variation; Gladiolus; histochemistry; leaves; localization; Methods. Procedures. Technologies; Modification of gene expression level; neoplasms; roots; transgenic plants; Iridaceae; Monocotyledones; Vegetals; β-Glucuronidase; Genetic transformation; Enzyme; Agrobacterium tumefaciens; Tissue culture; Tissue specificity; Biosynthesis; Gladiolus; Gene expression; Opine; Glycosidases; Angiospermae; Bacteria; Hydrolases; Flower crop; Spermatophyta; Rhizobiaceae; O-Glycosidases
• ISSN: 0721-7714; 1432-203X
• DOI: 10.1007/s002990050245

Five tumorigenic strains of Agrobacterium tumefaciens were used to inoculate corms, cormels, plants grown in vitro, and seed-derived seedlings of several cultivars of Gladiolus. Tumors formed on 12% of the plant tissues inoculated, and 1% of these tumors synthesized either octopine or nopaline. A. tumefaciens-mediated beta-glucuronidase (GUS) expression showed 0.5% and 3.5% GUS expression for plants grown in vitro and regenerable callus, respectively. GUS expression ranged from 40% to 61% when A. tumefaciens was incubated with leaves from seedlings grown in the dark, whereas leaves from seedlings grown under a 16-h light photoperiod showed no GUS, indicating the significant effect of etiolation on transient GUS expression mediated by A. tumefaciens.