Immobilized-metal affinity chromatography (IMAC): a review

This article reviews the development of immobilized-metal affinity chromatography (IMAC) and describes its most important applications. We provide an overview on the use of IMAC in protein fractionation and proteomics, in protein immobilization and detection, and on some special applications such as...

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Bibliographic Details
Published inMethods in enzymology Vol. 463; p. 439
Main Authors Block, Helena, Maertens, Barbara, Spriestersbach, Anne, Brinker, Nicole, Kubicek, Jan, Fabis, Roland, Labahn, Jörg, Schäfer, Frank
Format Journal Article
LanguageEnglish
Published United States 2009
Subjects
Animals
Automation - instrumentation
Automation - methods
Chromatography, Affinity - instrumentation
Chromatography, Affinity - methods
Equipment Reuse
Humans
Metals - chemistry
Metals - metabolism
Models, Biological
Protein Binding
Proteins - chemistry
Proteins - isolation & purification
Proteins - metabolism
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Summary:This article reviews the development of immobilized-metal affinity chromatography (IMAC) and describes its most important applications. We provide an overview on the use of IMAC in protein fractionation and proteomics, in protein immobilization and detection, and on some special applications such as purification of immunoglobulins and the Chelex method. The most relevant application-purification of histidine-tagged recombinant proteins-will be reviewed in greater detail with focus of state-of-the-art materials, methods, and protocols, and the limitations of IMAC and recent advances to improve the technology and the methods will be described.
ISSN:1557-7988
DOI:10.1016/S0076-6879(09)63027-5