Specific 125I radioimmunoassay for morphine

In this specific radioimmunoassay procedure for morphine, samples are first subjected to an extraction procedure to remove the major metabolite of morphine (morphine-3-glucuronide), then quantified by use of a commercial radioimmunoassay kit with 125I-labeled morphine. Concentrations between 10 and...

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Bibliographic Details
Published inClinical chemistry (Baltimore, Md.) Vol. 32; no. 1; pp. 157 - 158
Main Authors EDWARDS, D. J, POPOVSKI, Z, BAUMANN, T. J, BIVINS, B. A
Format Journal Article
LanguageEnglish
Published Washington, DC American Association for Clinical Chemistry 1986
Subjects
Analysis
Applied sciences
Biological and medical sciences
Exact sciences and technology
General pharmacology
Humans
Medical sciences
Morphine - blood
Other techniques and industries
Pharmacology. Drug treatments
Radioimmunoassay
Reagent Kits, Diagnostic
Assay
Serum
Narcotic analgesic
Metabolite
Radioimmunoassay
Immunological method
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Summary:In this specific radioimmunoassay procedure for morphine, samples are first subjected to an extraction procedure to remove the major metabolite of morphine (morphine-3-glucuronide), then quantified by use of a commercial radioimmunoassay kit with 125I-labeled morphine. Concentrations between 10 and and 100 micrograms/L are routinely measured with a within-run CV of less than 2.0% and a between-run CV of 3.5%. This rapid, specific, and sensitive method requires minimal technical skills and analytical equipment that is common to most laboratories.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
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ISSN:0009-9147
1530-8561
DOI:10.1093/clinchem/32.1.157