Specific 125I radioimmunoassay for morphine
In this specific radioimmunoassay procedure for morphine, samples are first subjected to an extraction procedure to remove the major metabolite of morphine (morphine-3-glucuronide), then quantified by use of a commercial radioimmunoassay kit with 125I-labeled morphine. Concentrations between 10 and...
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Published in | Clinical chemistry (Baltimore, Md.) Vol. 32; no. 1; pp. 157 - 158 |
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Main Authors | , , , |
Format | Journal Article |
Language | English |
Published |
Washington, DC
American Association for Clinical Chemistry
1986
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Subjects | |
Online Access | Get full text |
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Summary: | In this specific radioimmunoassay procedure for morphine, samples are first subjected to an extraction procedure to remove the major metabolite of morphine (morphine-3-glucuronide), then quantified by use of a commercial radioimmunoassay kit with 125I-labeled morphine. Concentrations between 10 and and 100 micrograms/L are routinely measured with a within-run CV of less than 2.0% and a between-run CV of 3.5%. This rapid, specific, and sensitive method requires minimal technical skills and analytical equipment that is common to most laboratories. |
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Bibliography: | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
ISSN: | 0009-9147 1530-8561 |
DOI: | 10.1093/clinchem/32.1.157 |