HPLC analysis, cytotoxicity, and safety study of Moringa oleifera Lam. (wild type) leaf extract

• Published in: Journal of food biochemistry Vol. 44; no. 10; pp. e13400 - n/a
• Main Authors: Saleem, Ammara, Saleem, Mohammad, Akhtar, Muhammad Furqan, Ashraf Baig, Mirza Muhammad Faran, Rasul, Azhar
• Format: Journal Article
• Language: English
• Published: United States 01.10.2020
• Subjects: acute toxicity; Moringa oleifera; oxidative stress; subchronic toxicity; subchronic toxicity; Moringa oleifera; acute toxicity; oxidative stress
• ISSN: 0145-8884; 1745-4514; 1745-4514
• DOI: 10.1111/jfbc.13400

The study was planned to evaluate toxicity of M. oleifera leaf methanol extract in Wistar rats, cytotoxic potential and chemically characterize it. Acute toxicity study revealed no mortality at 2,000 mg/kg dose. In subchronic toxicity, 150, 300, and 600 mg/kg extract were administered in both sexes for 90 days. A decrease in body weight, cholesterol, and low‐density lipoproteins, as well as an increase in the platelet count were observed. The histology of heart, lung, and kidney was normal. The oxidative stress biomarkers were normal in the liver tissue. In vitro cytotoxicity assay revealed that IC50 of the plant extract was more than 1,000 µg/ml. FTIR explored various functional groups and HPLC analysis indicated the presence of kaempferol and quercetin in the plant extract. These results showed that the plant extract might be safe up to 2,000 mg/kg single dose. The long term use of the plant extract was mostly devoid of major system toxicities. Practical applications M. oleifera is a multipurpose tree. It is a cruciferous plant that has nutritional as well as medicinal properties. It is exhibited a multitude of nutraceutical or pharmacological properties such as anti‐inflammatory, lipid‐lowering, anticancer, hypoglycemic, and antihypertensive activities. It is widely used by local inhabitants for their health, food, agriculture, and cosmetic purposes. Among other plant parts, its leaves are mostly used as a functional food because of abundant proteins, β‐carotene, polyphenol, vitamin C, and vitamin E. The current study was designed to validate its safety in acute and long term use. The data of this study suggested that the plant can be safely used as a nutraceutical as well as a nutritional food. The FTIR explored various functional groups and HPLC analysis indicated the presence of kaempferol and quercetin in the methanol extract of Moringa oleifera leaf. In vitro cytotoxicity assay revealed that IC50 of the plant extract was more than 1,000 µg/ml. The plant extract might be safe up to 2,000 mg/kg single dose. Long term use of the plant extract was mostly devoid of major system toxicities.