Single capture bright field and off-axis digital holographic microscopy

We report on a single capture approach for simultaneous incoherent bright field (BF) and laser-based quantitative phase imaging (QPI). Common-path digital holographic microscopy (DHM) is implemented in parallel with BF imaging within the optical path of a commercial optical microscope to achieve spa...

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Bibliographic Details
Published inOptics letters Vol. 48; no. 4; p. 876
Main Authors Picazo-Bueno, José Ángel, Barroso, Álvaro, Ketelhut, Steffi, Schnekenburger, Jürgen, Micó, Vicente, Kemper, Björn
Format Journal Article
LanguageEnglish
Published United States 15.02.2023
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Summary:We report on a single capture approach for simultaneous incoherent bright field (BF) and laser-based quantitative phase imaging (QPI). Common-path digital holographic microscopy (DHM) is implemented in parallel with BF imaging within the optical path of a commercial optical microscope to achieve spatially multiplexed recording of white light images and digital off-axis holograms, which are subsequently numerically demultiplexed. The performance of the proposed multimodal concept is firstly determined by investigations on microspheres. Then, the application for label-free dual-mode QPI and BF imaging of living pancreatic tumor cells is demonstrated.
ISSN:1539-4794
DOI:10.1364/OL.478674