Improved fluorometric method to assay for soil lipase activity

Optimum conditions for the precise assay of soil lipases are described, using the fluorogenic substrate 4-methyl umbelliferone nonanoate (4MUN). The method utilizes a 3 h 0.1 m tetra-sodium pyrophosphate (pH 7.5) extraction, followed by a 10 min assay. Soil lipase is measured with average 95% confid...

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Bibliographic Details
Published inSoil biology & biochemistry Vol. 13; no. 4; pp. 307 - 311
Main Authors Cooper, A.B., Morgan, H.W.
Format Journal Article
LanguageEnglish
Published Elsevier Ltd 1981
Subjects
soil chemistry
soil physics
soil science
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Summary:Optimum conditions for the precise assay of soil lipases are described, using the fluorogenic substrate 4-methyl umbelliferone nonanoate (4MUN). The method utilizes a 3 h 0.1 m tetra-sodium pyrophosphate (pH 7.5) extraction, followed by a 10 min assay. Soil lipase is measured with average 95% confidence limits of 5.6%. Activity was optimal between 30–40°C. Soil extracts characteristically possessed two pH-activity peaks, one each side of neutrality. Extracts were stable when stored at 4°C for at least 5 days.
ISSN:0038-0717
1879-3428
DOI:10.1016/0038-0717(81)90067-5