Peroxidase in plant defense: Novel insights for cadmium accumulation in rice (Oryza sativa L.)

• Published in: Journal of hazardous materials Vol. 474; p. 134826
• Main Authors: Liu, Jiahui, Lv, Yunxuan, Li, Meng, Wu, Yingjie, Li, Bing, Wang, Changquan, Tao, Qi
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 05.08.2024
• Subjects: Cadmium; Lignin; Membranal metal transporters; Peroxidase; Phenylpropanoid biosynthesis; Rice plant; Cadmium; Lignin; Phenylpropanoid biosynthesis; Peroxidase; Membranal metal transporters; Rice plant
• ISSN: 0304-3894; 1873-3336; 1873-3336
• DOI: 10.1016/j.jhazmat.2024.134826

Phenylpropanoid biosynthesis plays crucial roles in the adaptation to cadmium (Cd) stress. Nevertheless, few reports have dabbled in physiological mechanisms of such super pathway regulating Cd accumulation in plants. Herein, by integrating transcriptomic, histological and molecular biology approaches, the present study dedicated to clarify molecular mechanism on how rice adapt to Cd stress via phenylpropanoid biosynthesis. Our analysis identified that the enhancement of phenylpropanoid biosynthesis was as a key response to Cd stress. Intriguingly, POD occupied a significant part in this process, with the number of POD related genes accounted for 26/29 of all upregulated genes in phenylpropanoid biosynthesis. We further used SHAM (salicylhydroxamic acid, the POD inhibitor) to validate that POD exhibited a negative correlation with the Cd accumulation in rice tissues, and proposed two intrinsic molecular mechanisms on POD in contributing to Cd detoxification. One strategy was that POD promoted the formation of lignin and CSs both in endodermis and exodermis for intercepting Cd influx. In detail, inhibited POD induced by external addition of SHAM decreased the content of lignin by 50.98–66.65 % and delayed percentage of the DTIP-CS to root length by 39.17–104.51 %. The other strategy was expression of transporter genes involved in Cd uptake, including OsIRT1, OsIRT2, OsZIP1 and OsZIP, negatively regulated by POD. In a word, our findings firstly draws a direct link between POD activity and the Cd accumulation, which is imperative for the breeding of rice with low-Cd-accumulating capacity in the future. [Display omitted] •26 of 29 upregulated genes in phenylpropanoid biosynthesis are associated with POD.•POD negatively regulate Cd uptake and accumulation.•POD involve in promoting the formation of lignin and CSs in endodermis and exodermis for Cd detoxification.•POD involve in orchestrating the membranal metal transporters on Cd detoxification.