Improving the stability of insulin through effective chemical modifications: A Comprehensive review

• Published in: International journal of pharmaceutics Vol. 661; p. 124399
• Main Authors: Kamelnia, Reyhane, Ahmadi-hamedani, Mahmood, Darroudi, Majid, Kamelnia, Elahe
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 15.08.2024
• Subjects: Aggregation; Chemical modification; In vivo; Insulin; Nanoparticle; ThT; NP; SDS; SPIONs; Nanoparticle; T1D; IGF2AS; T2D; DM; MSN; GNPs; Insulin; CTAB; PTMs; Aggregation; In vivo; B(OH)3; Chemical modification; CAs; TH; BPB; NCL; STZ; AC INS
• ISSN: 0378-5173; 1873-3476; 1873-3476
• DOI: 10.1016/j.ijpharm.2024.124399

[Display omitted] Insulin, an essential peptide hormone, conjointly regulates blood glucose levels by its receptor and it is used as vital drug to treat diabetes. This therapeutic hormone may undergo different chemical modifications during industrial processes, pharmaceutical formulation, and through its endogenous storage in the pancreatic β-cells. Insulin is highly sensitive to environmental stresses and readily undergoes structural changes, being also able to unfold and aggregate in physiological conditions. Even; small changes altering the structural integrity of insulin may have significant impacts on its biological efficacy to its physiological and pharmacological activities. Insulin analogs have been engineered to achieve modified properties, such as improved stability, solubility, and pharmacokinetics, while preserving the molecular pharmacology of insulin. The casually or purposively strategies of chemical modifications of insulin occurred to improve its therapeutic and pharmaceutical properties. Knowing the effects of chemical modification, formation of aggregates, and nanoparticles on protein can be a new look at the production of protein analogues drugs and its application in living system. The project focused on effects of chemical modifications and nanoparticles on the structure, stability, aggregation and their results in effective drug delivery system, biological activity, and pharmacological properties of insulin. The future challenge in biotechnology and pharmacokinetic arises from the complexity of biopharmaceuticals, which are often molecular structures that require formulation and delivery strategies to ensure their efficacy and safety.